Evidence map›Paper›PMID 33997801›Full record

ArticleSTAR protocols2021

A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples.

Anita Shete, Sreelekshmy Mohandas, Rajlaxmi Jain, Pragya D Yadav

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Monkeypox: An emerging zoonotic pathogen.World journal of virology · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Anita SheteIndian Council of Medical Research-National Institute of Virology, Pune, Maharashtra 411021, India.
Sreelekshmy MohandasIndian Council of Medical Research-National Institute of Virology, Pune, Maharashtra 411021, India.
Rajlaxmi JainIndian Council of Medical Research-National Institute of Virology, Pune, Maharashtra 411021, India.
Pragya D YadavIndian Council of Medical Research-National Institute of Virology, Pune, Maharashtra 411021, India.
ICMR-National Institute of Virology · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigens and the negative control antigen and the use of antigen-coated microtiter plates to detect SARS-CoV-2-specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive versus negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently prepared antigens. For complete details on the use and execution of this protocol, please refer to Mohandas et al. (2021).

Indexed as

AnimalsAntibodies, ViralCoronavirus Nucleocapsid ProteinsCOVID-19Enzyme-Linked Immunosorbent AssayImmunoglobulin GImmunologic TestsMesocricetusSARS-CoV-2Antibodies, ViralCoronavirus Nucleocapsid ProteinsImmunoglobulin GAntibodyImmunologyMicrobiologyModel Organisms

Identifiers

PMID33997801
PMCPMC8108381
OpenAlexW3160669335

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.