ArticleInternational journal of reproductive biomedicine2021
Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study.
Article in International journal of reproductive biomedicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 8 citations in OpenAlex.
- Bioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization.Advances in experimental medicine and biology · 2025Review
- Retinoic acid-releasing scaffold based on chitosan hydrogel and testis decellular plates.BioImpacts : BI · 2025Article
- Influence of Storage Conditions on Decellularized Porcine Conjunctiva.Bioengineering (Basel, Switzerland) · 2023Article
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Engineering Dynamic 3D Models of Lung.Advances in experimental medicine and biology · 2023Article
- Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions.Cancers · 2022Review
- Decellularization of xenografted tumors provides cell-specificFrontiers in oncology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiological scaffolds are derived by the decellularization of tissues or organs. Various biological scaffolds, such as scaffolds for the liver, lung, esophagus, dermis, and human testicles, have been produced. Their application in tissue engineering has created the need for cryopreservation processes to store these scaffolds.
objectiveThe aim was to compare the two methods for prolong storage testicular scaffolds. MATERIALS AND
methodsIn this experimental study, 20 male NMRI mice (8 wk) were sacrificed and their testes were removed and treated with 0.5% sodium dodecyl sulfate followed by Triton X-100 0.5%. The efficiency of decellularization was determined by histology and DNA quantification. Testicular scaffolds were stored in phosphate-buffered saline solution at 4°C or cryopreserved by programmed slow freezing followed by storage in liquid nitrogen. Masson's trichrome staining, Alcian blue staining and immunohistochemistry, collagen assay, and glycosaminoglycan assay were done prior to and after six months of storage under each condition.
resultsHematoxylin-eosin staining showed no remnant cells after the completion of decellularization. DNA content analysis indicated that approximately 98% of the DNA was removed from the tissue (p = 0.02). Histological evaluation confirmed the preservation of extracellular matrix components in the fresh and frozen-thawed scaffolds. Extracellular matrix components were decreased by 4°C-stored scaffolds. Cytotoxicity tests with mouse embryonic fibroblast showed that the scaffolds were biocompatible and did not have a harmful effect on the proliferation of mouse embryonic fibroblast cells.
conclusionOur results demonstrated the superiority of the slow freezing method for prolong storage of testicular scaffolds.
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