Evidence map›Paper›PMID 33997514›Full record

ArticleBioactive materials2021

Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells.

Bin Jiang, Weijie Li, Samantha Stewart, Wenquan Ou, Baolin Liu, Pierre Comizzoli, Xiaoming He

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioactive materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
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  5. Review
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  7. Article
  8. A highly active mineral-based ice nucleating agent supportsJournal of the Royal Society, Interface · 2023
    Article
  9. Review
  10. Article
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  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Bin JiangFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Weijie LiFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Samantha StewartFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Wenquan OuFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Baolin LiuInstitute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Pierre ComizzoliSmithsonian Conservation Biology Institute, National Zoological Park, Washington, DC, 20008, USA.
Xiaoming HeFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
University of Maryland, College Park · USUniversity of Shanghai for Science and Technology · CNSmithsonian Conservation Biology Institute · USUniversity of Maryland, Baltimore · US

Funding

Microfluidic encapsulation of ovarian follicles for biomimetic 3D culture andcryopreservationR01EB023632 · NIBIB · UNIV OF MARYLAND, COLLEGE PARK · PI HE, XIAOMING · 2017 to 2020
$1.6M
NIBIB NIH HHS R01 EB023632
6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. Here, sand from nature is discovered to be capable of seeding ice above -10 °C, which enables cryopreservation of hiPSCs with no serum, much-reduced cryoprotectant, and high cell survival. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.

Indexed as

CryopreservationIce seedingiPSCSandStem cell

Identifiers

PMID33997514
PMCPMC8111032
OpenAlexW3162602453

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.