Evidence map›Paper›PMID 33997506›Full record

ArticleBioactive materials2021

Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels.

Zhiyuan Jia, Mareike Müller, Tony Le Gall, Martijn Riool, Max Müller, Sebastian A J Zaat, Tristan Montier, Holger Schönherr

Open access · goldAbstract read
In one paragraph

Article in Bioactive materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Smart Hydrogels in Wearable Electronics for Wound Treatments.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Review
  5. Review
  6. Wireless Intelligent Patch for Closed-loop In Situ Wound Management.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  7. Article
  8. Article
  9. From Chitosan to Chitin: Bio-Inspired Thin Films for Passive Daytime Radiative Cooling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  10. Review
  11. Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 5 countries.

Zhiyuan JiaPhysical Chemistry I & Research Center of Micro and Nanochemistry and Engineering (), Department of Chemistry and Biology, University of Siegen, Adolf-Reichwein-Straße 2, 57076, Siegen, Germany.
Mareike MüllerPhysical Chemistry I & Research Center of Micro and Nanochemistry and Engineering (), Department of Chemistry and Biology, University of Siegen, Adolf-Reichwein-Straße 2, 57076, Siegen, Germany.
Tony Le GallUniv Brest, INSERM, EFS, UMR 1078 GGFB, F-29200, Brest, France.
Martijn RioolDepartment of Medical Microbiology and Infection Prevention, Amsterdam institute for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
Max MüllerPhysical Chemistry I & Research Center of Micro and Nanochemistry and Engineering (), Department of Chemistry and Biology, University of Siegen, Adolf-Reichwein-Straße 2, 57076, Siegen, Germany.
Sebastian A J ZaatDepartment of Medical Microbiology and Infection Prevention, Amsterdam institute for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
Tristan MontierUniv Brest, INSERM, EFS, UMR 1078 GGFB, F-29200, Brest, France.
Holger SchönherrPhysical Chemistry I & Research Center of Micro and Nanochemistry and Engineering (), Department of Chemistry and Biology, University of Siegen, Adolf-Reichwein-Straße 2, 57076, Siegen, Germany.
University of Siegen · DEAmsterdam University Medical Centers · NLInserm · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid, sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme reactions. These patterned sensor hydrogels are functionalized with three different colorimetric enzyme substrates affording the multiplexed detection and differentiation of α-glucosidase, β-galactosidase and β-glucuronidase. The limits of detection of the hydrogels for an observation time of 60 min using a conventional microplate reader correspond to concentrations of 0.2, 3.4 and 4.5 nM of these enzymes, respectively. Based on their different enzyme expression patterns,

Indexed as

Bacteria detectionBacterial enzymeColorimetric substratesMultiplexed biosensorsReporter hydrogels

Identifiers

PMID33997506
PMCPMC8105640
OpenAlexW3160863507

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.