ArticleFrontiers in cell and developmental biology2021
Mouse Retinal Organoid Growth and Maintenance in Longer-Term Culture.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Accessory Inner Segment-Like Structures Develop in Human Retinal Organoids.Stem cell reviews and reports · 2026Article
- Modulation of Systemic Osmolarity Alters Retinal Thickness and Schisis Cavities Without Blood Retinal Barrier Disruption in Rs1 Knockout Mice.Investigative ophthalmology & visual science · 2026Article
- Blocking apoptosis promotes survival and alters developmental dynamics of human retinal ganglion cells in retinal organoids.Cell reports · 2026Article
- Characterization of age-related dolichol increases in the retina of the C57BL/6 mouse.Scientific reports · 2025Article
- Robust generation of photoreceptor-dominant retinal organoids from porcine induced pluripotent stem cells.Stem cell reports · 2025Article
- DNA Origami Barcodes for Immunostaining.ACS applied materials & interfaces · 2025Article
- Bridging the gap: the role of 3D cell cultures in mimicking tumor microenvironment for enhanced drug testing accuracy.Frontiers in bioengineering and biotechnology · 2025Review
- Automated quantification of photoreceptor outer segments in developing and degenerating retinas on microscopy images across scales.Frontiers in molecular neuroscience · 2024Article
- Intrinsic signal optoretinography of dark adaptation abnormality due to rod photoreceptor degeneration.Experimental biology and medicine (Maywood, N.J.) · 2024Article
- Deletion of IFT20 exclusively in the RPE ablates primary cilia and leads to retinal degeneration.PLoS biology · 2023Article
- Mathematical model for glutathione dynamics in the retina.Scientific reports · 2023Article
- Reliability of human retina organoid generation from hiPSC-derived neuroepithelial cysts.Frontiers in cellular neuroscience · 2023Article
- Modeling inducible neuropathologies of the retina with differential phenotypes in organoids.Frontiers in cellular neuroscience · 2023Article
- HBEGF-TNF induce a complex outer retinal pathology with photoreceptor cell extrusion in human organoids.Nature communications · 2022Article
- Transplanted human cones incorporate into the retina and function in a murine cone degeneration model.The Journal of clinical investigation · 2022Article
- Spata7 is required for maintenance of the retinal connecting cilium.Scientific reports · 2022Article
- Review
- Sequential in vivo labeling of insulin secretory granule pools inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Prospects for Retinal Organoids in Treatment of Retinal Diseases.Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using retinal organoid systems, organ-like 3D tissues, relies implicitly on their robustness. However, essential key parameters, particularly retinal growth and longer-term culture, are still insufficiently defined. Here, we hypothesize that a previously optimized protocol for high yield of evenly-sized mouse retinal organoids with low variability facilitates assessment of such parameters. We demonstrate that these organoids reliably complete retinogenesis, and can be maintained at least up to 60 days in culture. During this time, the organoids continue to mature on a molecular and (ultra)structural level: They develop photoreceptor outer segments and synapses, transiently maintain its cell composition for about 5-10 days after completing retinogenesis, and subsequently develop pathologic changes - mainly of the inner but also outer retina and reactive gliosis. To test whether this organoid system provides experimental access to the retina during and upon completion of development, we defined and stimulated organoid growth by activating sonic hedgehog signaling, which in patients and mice
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.