Evidence map›Paper›PMID 33996243›Full record

ArticleBiomedical optics express2021

Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.

Michael L Buttolph, Menansili A Mejooli, Pavel Sidorenko, Chi-Yong Eom, Chris B Schaffer, Frank W Wise

Abstract read
In one paragraph

Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Circumscribing Laser Cuts Attenuate Seizure Propagation in a Mouse Model of Focal Epilepsy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Michael L ButtolphSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.ORCID https://orcid.org/0000-0001-8387-5299
Menansili A MejooliMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
Pavel SidorenkoSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.ORCID https://orcid.org/0000-0002-7161-4267
Chi-Yong EomMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
Chris B SchafferMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
Frank W WiseSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Funding

Tissue regulation of T cell function - Reagents CoreP01AI102851 · NIAID · UNIVERSITY OF ROCHESTER · PI Iwijn De Vlaminck · 2014 to 2026
$26.0M
NIAID NIH HHS P01 AI102851
6 · The paper itself

Abstract

Two-photon fluorescence microscopy is a nonlinear imaging modality frequently used in deep-tissue imaging applications. A tunable-wavelength multicolor short-pulse source is usually required to excite fluorophores with a wide range of excitation wavelengths. This need is most typically met by solid-state lasers, which are bulky, expensive, and complicated systems. Here, we demonstrate a compact, robust fiber system that generates naturally synchronized femtosecond pulses at 1050 nm and 1200 nm by using a combination of gain-managed and Raman amplification. We image the brain of a mouse and view the blood vessels, neurons, and other cell-like structures using simultaneous degenerate and nondegenerate excitation.

Identifiers

PMID33996243
PMCPMC8086478

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.