ArticleBiomedical optics express2021
Single-scan HiLo with line-illumination strategy for optical section imaging of thick tissues.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Superresolution structured illumination microscopy reconstruction algorithms: a review.Light, science & applications · 2023Review
- 3D autofluorescence imaging of hydronephrosis and renal anatomical structure using cryo-micro-optical sectioning tomography.Theranostics · 2023Article
- Cryo-fluorescence micro-optical sectioning tomography for volumetric imaging of various whole organs with subcellular resolution.iScience · 2022Article
- High-speed mosaic imaging using scanner-synchronized stage position sampling.Journal of biomedical optics · 2022Article
- HiLo Based Line Scanning Temporal Focusing Microscopy for High-Speed, Deep Tissue Imaging.Membranes · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical sectioning has been widely employed for inhibiting out-of-focus backgrounds in three-dimensional (3D) imaging of biological samples. However, point scanning imaging or multiple acquisitions for wide-field optical sectioning in epi-illumination microscopy remains time-consuming for large-scale imaging. In this paper, we propose a single-scan optical sectioning method based on the hybrid illumination (HiLo) algorithm with a line-illumination strategy. Our method combines HiLo background inhibition with confocal slit detection. It thereby offers a higher optical sectioning capability than wide-field HiLo and line-confocal imaging without extra modulation and multiple data acquisition. To demonstrate the optical-sectioning capability of our system, we imaged a thin fluorescent plane and different fluorescence-labeled mouse tissue. Our method shows an excellent background inhibition in thick tissue and thus potentially provides an alternative tool for 3D imaging of large-scale biological tissue.
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Registered trials
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