ArticleBiomedical optics express2021
Open-top axially swept light-sheet microscopy.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Widefield Targeted Illumination Microscopy Enables Optically-Sectioned, Motion-Resilient Imaging of Neuronal Fibers and Their Dynamics.Laser & photonics reviews · 2025Article
- Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology.Nature methods · 2025Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Light-sheet microscopy enabled by a miniaturized plane illuminator.Biomedical optics express · 2025Article
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Mesoscopic axially swept oblique plane microscope for the imaging of freely moving organisms with near-isotropic resolution.Biomedical optics express · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Axially swept open-top light-sheet microscopy for densely labeled clinical specimens.Optics letters · 2024Article
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
- High-resolution open-top axially swept light sheet microscopy.BMC biology · 2023Article
- Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry.Lab on a chip · 2023Article
- Practical considerations for quantitative light sheet fluorescence microscopy.Nature methods · 2022Review
- Deep learning enables reference-free isotropic super-resolution for volumetric fluorescence microscopy.Nature communications · 2022Article
- DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.Nature methods · 2022Article
- Axial resolution and imaging contrast enhancement in inverted light-sheet microscopy by natural illumination modulation.Frontiers in neuroscience · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for high throughput cellular imaging of large tissue specimens including optically cleared tissues by having the entire optical setup below the sample stage. Current OT-LSM systems had relatively low axial resolutions by using weakly focused light sheets to cover the imaging field of view (FOV). In this report, open-top axially swept LSM (OTAS-LSM) was developed for high-throughput cellular imaging with improved axial resolution. OTAS-LSM swept a tightly focused excitation light sheet across the imaging FOV using an electro tunable lens (ETL) and collected emission light at the focus of the light sheet with a camera in the rolling shutter mode. OTAS-LSM was developed by using air objective lenses and a liquid prism and it had on-axis optical aberration associated with the mismatch of refractive indices between air and immersion medium. The effects of optical aberration were analyzed by both simulation and experiment, and the image resolutions were under 1.6µm in all directions. The newly developed OTAS-LSM was applied to the imaging of optically cleared mouse brain and small intestine, and it demonstrated the single-cell resolution imaging of neuronal networks. OTAS-LSM might be useful for the high-throughput cellular examination of optically cleared large tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.