Evidence map›Paper›PMID 33995452›Full record

ArticleFrontiers in plant science2021

Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease Digestion.

Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S Pikaard

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Anastasia McKinlayDepartment of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States.
Dalen FultzDepartment of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States.
Feng WangDepartment of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States.
Craig S PikaardDepartment of Biology and Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States.
Howard Hughes Medical Institute · USIndiana University Bloomington · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large regions of nearly identical repeats, such as the 45S ribosomal RNA (rRNA) genes of Nucleolus Organizer Regions (NORs), can account for major gaps in sequenced genomes. To assemble these regions, ultra-long sequencing reads that span multiple repeats have the potential to reveal sets of repeats that collectively have sufficient sequence variation to unambiguously define that interval and recognize overlapping reads. Because individual repetitive loci typically represent a small proportion of the genome, methods to enrich for the regions of interest are desirable. Here we describe a simple method that achieves greater than tenfold enrichment of

Indexed as

Arabidopsis thalianaNORNucleolus Organizer RegionOxford Nanopore sequencingribosomal RNA gene enrichment

Identifiers

PMID33995452
PMCPMC8113872
OpenAlexW3159783900

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.