Evidence map›Paper›PMID 33995301›Full record

ArticleFrontiers in microbiology2021

Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis.

Shohei Yasuda, Toshikazu Suenaga, Laura Orschler, Shelesh Agrawal, Susanne Lackner, Akihiko Terada

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shohei YasudaDepartment of Chemical Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
Toshikazu SuenagaGlobal Innovation Research Institute, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Laura OrschlerDepartment of Civil and Environmental Engineering Science, Institute IWAR, Chair of Wastewater Engineering, Technical University of Darmstadt, Darmstadt, Germany.
Shelesh AgrawalDepartment of Civil and Environmental Engineering Science, Institute IWAR, Chair of Wastewater Engineering, Technical University of Darmstadt, Darmstadt, Germany.
Susanne LacknerDepartment of Civil and Environmental Engineering Science, Institute IWAR, Chair of Wastewater Engineering, Technical University of Darmstadt, Darmstadt, Germany.
Akihiko TeradaDepartment of Chemical Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Upcycling wastes into valuable products by mixed microbial communities has recently received considerable attention. Sustainable production of high-value substances from one-carbon (C1) compounds, e.g., methanol supplemented as an external electron donor in bioreactors for wastewater treatment, is a promising application of upcycling. This study undertook a gene-centric approach to screen valuable production potentials from mixed culture biomass, removing organic carbon and nitrogen from landfill leachate. To this end, the microbial community of the activated sludge from a landfill leachate treatment plant and its metabolic potential for the production of seven valuable products were investigated. The DNA extracted from the activated sludge was subjected to shotgun metagenome sequencing to analyze the microbial taxonomy and functions associated with producing the seven products. The functional analysis confirmed that the activated sludge could produce six of the valuable products, ectoine, polyhydroxybutyrate (PHB), zeaxanthin, astaxanthin, acetoin, and 2,3-butanediol. Quantification of the detected functional gene hit numbers for these valuable products as a primary trial identified a potential rate-limiting metabolic pathway, e.g., conversion of

Indexed as

activated sludgelandfillleachatemetagenomemetagenomic screeningMethyloceanibacter

Identifiers

PMID33995301
PMCPMC8121002

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.