ReviewJournal of physics D: Applied physics2020
Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.
Review in Journal of physics D: Applied physics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Phototoxicity of brightfield live-cell imaging on murine ovarian follicles.Archives of gynecology and obstetrics · 2026Article
- The microtubule GTP-tubulin cap size is modulated during cell division.Molecular biology of the cell · 2026Article
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- The microtubule GTP-tubulin cap size is modulated during cell division.bioRxiv : the preprint server for biology · 2026Article
- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Review
- Enhancing ultra-high density single-molecule localization with deep spatiotemporal networks.Biomedical optics express · 2025Article
- The Matrix of Mitochondrial Imaging: Exploring Spatial Dimensions.Biomolecules · 2025Review
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Gentle Rhodamines for Live-Cell Fluorescence Microscopy.ACS central science · 2024Article
- Photothermal Excitation of Neurons Using MXene: Cellular Stress and Phototoxicity Evaluation.Advanced healthcare materials · 2024Article
- Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling.Nature communications · 2024Article
- Harnessing artificial intelligence to reduce phototoxicity in live imaging.Journal of cell science · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- Photobleaching and phototoxicity of mitochondria in live cell fluorescent super-resolution microscopy.Mitochondrial communications · 2024Article
- Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging.Neurophotonics · 2024Article
- An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging.Communications biology · 2023Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
- Label-free microscopy for virus infections.Microscopy (Oxford, England) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Super-resolution microscopy (SRM) enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we give an overview of new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.