Evidence map›Paper›PMID 33993387›Full record

ArticleEcoHealth2020

Isolation and Genomic Characterization of Echovirus 11 from faeces of a Non-Human Primate in Nigeria.

T O C Faleye, U E George, D Klapsa, M Majumdar, A O Oragwa, M O Adewumi, J Martin, J A Adeniji

Abstract read
PubMed Publisher
In one paragraph

Article in EcoHealth, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

T O C FaleyeDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo, Nigeria.
U E GeorgeDepartment of Biological Sciences, Redeemer's University, Ede, Nigeria.
D KlapsaDivision of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, UK.
M MajumdarDivision of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, UK.
A O OragwaDepartment of Veterinary Microbiology and Pathology, Faculty of Veterinary Medicine, University of Jos, Jos, Nigeria.
M O AdewumiDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo, Nigeria. adewumi1@hotmail.com.ORCID http://orcid.org/0000-0002-5172-5808
J MartinDivision of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, UK.
J A AdenijiDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo, Nigeria.
National Institute for Biological Standards and Control · GBUniversity of Ibadan · NGRedeemer's University · NGUniversity of Jos · NG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We recently investigated the presence of enteroviruses (EVs) in non-human primates (NHPs) in Northern Nigeria and documented the presence of EV-A76 of South-East Asian ancestry in an NHP. In this study, we go further to ask if we could also find EVs in NHPs indigenous to the forested South-south Nigeria. Fresh faecal samples were collected from the floor of 10 cages housing NHPs in Cross River Nigeria, re-suspended in PBS and subjected to RNA extraction, cDNA synthesis, PanEnt 5'-UTR and PanEnt VP1 PCR assays. None of the samples was positive for the PanEnt VP1 assay, but one sample was positive for PanEnt 5'-UTR PCR. This sample was subsequently inoculated into RD cell line, produced CPE and the isolate analysed by PCR assays, next-generation whole genome sequencing and passage in four different cell lines showing replication in two of them. Analysis of the complete genome of the isolate identified it as an Echovirus 11 (E11) and revealed a recombinant genomic structure. Phylogenetic analysis showed that the E11 NHP strain was related to human clinical isolates suggesting a zoonotic behaviour. We describe the first isolation and complete genome characterization of an E11 obtained from an NHP in Nigeria having zoonotic potential.

Indexed as

Enterovirus B, HumanPrimatesAnimalsFecesGenomicsNigeriaPhylogenyComplete genomeEchovirus 11EnterovirusesNigeriaRD cell culture

Identifiers

PMID33993387
OpenAlexW3162361192

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.