Evidence map›Paper›PMID 33991846›Full record

ArticleBiosensors & bioelectronics2021

An integrated E-Tube cap for sample preparation, isothermal amplification and label-free electrochemical detection of DNA.

Zhiheng Xu, Kun Yin, Xiong Ding, Ziyue Li, Xuanhao Sun, Baikun Li, Rajesh V Lalla, Robert Gross, Changchun Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 24 citations in OpenAlex.

  1. Wireless IrOACS measurement science au · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Zhiheng XuDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Kun YinDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Xiong DingDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Ziyue LiDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Xuanhao SunDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, 06269, USA.
Baikun LiDepartment of Civil & Environmental Engineering, University of Connecticut, Storrs, CT, 06269, USA.
Rajesh V LallaSection of Oral Medicine, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Robert GrossDepartment of Medicine (Infectious Diseases), University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA; Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Changchun LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA. Electronic address: chaliu@uchc.edu.
UConn Health · USUniversity of Connecticut · USUniversity of Pennsylvania · US

Funding

Virus & Reservoirs CoreP30AI045008 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ROBERT GROSS · 1999 to 2026
$78.6M
Asymmetric CRISPR Approach for Nucleic Acid QuantificationR01EB023607 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI Changchun Liu · 2017 to 2026
$2.4M
Self-Powered Sample Concentrating and CRISPR-based Biosensing for Moile HIV-1 RNA DetectionR61AI154642 · NIAID · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI LIU, CHANGCHUN · 2020 to 2022
$1.4M
Point of Care Diagnostics of HPV-Associated Cervical Cancer in HIV Epidemic Areas in ChinaR01CA214072 · NCI · UNIVERSITY OF PENNSYLVANIA · PI LIU, CHANGCHUN · 2017 to 2021
$771k
NCI NIH HHS R01 CA214072NIAID NIH HHS P30 AI045008NIAID NIH HHS R61 AI154642NIBIB NIH HHS R01 EB023607
6 · The paper itself

Abstract

A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was designed, fabricated, and tested. The E-tube cap contains a 3D printed electrode substrate for DNA extraction and label-free pH sensing detection. One Flinders Technology Associates (Whatman FTA) membrane was incorporated into the 3D printed electrode substrate for the isolation, concentration, and purification of DNA. The E-tube cap with captured DNA by the membrane was inserted directly into a reaction tube for loop-mediated isothermal amplification (LAMP). The isothermal amplification process was monitored in real-time by a 3D printed electrochemical electrode coated with pH-sensitive material (carbon/iridium oxide layer). The pH sensing electrode showed an excellent linear response within the pH range of 6-9 with a slope of -31.32 ± 0.5 mV/pH at room temperature. The utility of the integrated E-tube cap was demonstrated by detecting the presence of lambda DNA spiked in saliva samples with a sensitivity of 100 copies per mL sample within 30 min. Such a simple, rapid, and affordable diagnostic device is particularly suitable for point-of-care molecular diagnostics of infectious diseases.

Indexed as

3D-printingElectrochemical detectionIsothermal amplification (LAMP)Loop-mediatedpH-sensitive electrodePoint-of-care molecular diagnostics

Identifiers

PMID33991846
PMCPMC8572321
OpenAlexW3161404700

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.