Evidence map›Paper›PMID 33990618›Full record

ArticleScientific data2021

Comprehensive dataset of shotgun metagenomes from oxygen stratified freshwater lakes and ponds.

Moritz Buck, Sarahi L Garcia, Leyden Fernandez, Gaëtan Martin, Gustavo A Martinez-Rodriguez, Jatta Saarenheimo, Jakob Zopfi, Stefan Bertilsson, Sari Peura

Abstract readDataset
In one paragraph

Article in Scientific data, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed.

  1. Article
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  6. MagnetotacticISME communications · 2026
    Article
  7. Article
  8. Article
  9. Article
  10. An organotrophicApplied and environmental microbiology · 2025
    Article
  11. Article
  12. Metagenome-assembled genome ofMicrobiology resource announcements · 2025
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
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  19. Analysis of metagenomic data.Nature reviews. Methods primers · 2025
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Moritz BuckDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Uppsala, Sweden. moritz.buck@slu.se.
Sarahi L GarciaDepartment of Ecology, Environment and Plant Sciences, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.ORCID 0000-0002-8622-0308
Leyden FernandezDepartment of Ecology and Genetics, Limnology, Uppsala University, Uppsala, Sweden.
Gaëtan MartinDepartment of Forest Mycology and Plant Pathology, Science for Life Laboratory, Swedish University of Agricultural Sciences, Uppsala, Sweden.ORCID 0000-0002-5289-6131
Gustavo A Martinez-RodriguezAgroenvironmental Science Department, University of Puerto Rico, Mayaguez Campus, San Juan, Puerto Rico, USA.
Jatta SaarenheimoDepartment of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
Jakob ZopfiDepartment of Environmental Sciences, Aquatic and Stable Isotope Biogeochemistry, University of Basel, Basel, Switzerland.ORCID 0000-0002-8437-7344
Stefan BertilssonDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Uppsala, Sweden.ORCID 0000-0002-4265-1835
Sari PeuraDepartment of Forest Mycology and Plant Pathology, Science for Life Laboratory, Swedish University of Agricultural Sciences, Uppsala, Sweden. sari.peura@slu.se.ORCID 0000-0003-3892-8157

Funding

Academy of Finland (Suomen Akatemia) 292251Svenska Forskningsrådet Formas (Swedish Research Council Formas) 2016-00874
6 · The paper itself

Abstract

Stratified lakes and ponds featuring steep oxygen gradients are significant net sources of greenhouse gases and hotspots in the carbon cycle. Despite their significant biogeochemical roles, the microbial communities, especially in the oxygen depleted compartments, are poorly known. Here, we present a comprehensive dataset including 267 shotgun metagenomes from 41 stratified lakes and ponds mainly located in the boreal and subarctic regions, but also including one tropical reservoir and one temperate lake. For most lakes and ponds, the data includes a vertical sample set spanning from the oxic surface to the anoxic bottom layer. The majority of the samples were collected during the open water period, but also a total of 29 samples were collected from under the ice. In addition to the metagenomic sequences, the dataset includes environmental variables for the samples, such as oxygen, nutrient and organic carbon concentrations. The dataset is ideal for further exploring the microbial taxonomic and functional diversity in freshwater environments and potential climate change impacts on the functioning of these ecosystems.

Indexed as

MetagenomeArchaeaBacteriaCarbon CycleClimate ChangeGreenhouse GasesLakesMicrobiotaOxygenPhylogenyPondsGreenhouse GasesOxygen

Identifiers

PMID33990618
PMCPMC8121793

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.