ArticleNPJ biofilms and microbiomes2021
Biofilm viability checker: An open-source tool for automated biofilm viability analysis from confocal microscopy images.
Article in NPJ biofilms and microbiomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed.
- Article
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- Ultrasound-Responsive Nanoparticles Enable Hydrophobic Antibiotic Release and Deep Penetration for Biofilm Treatment.JACS Au · 2026Article
- Investigating Salmonella biofilm responses to antibiotic treatment using optical photothermal infrared spectroscopy.Communications biology · 2026Article
- Impact of Modified Lactoperoxidase Systems on Glycolytic Metabolism and Virulence Factors inInternational journal of molecular sciences · 2026Article
- UnderstandingMicroorganisms · 2026Review
- Trimethyl chitosan: Antibacterial activity onJournal of dental sciences · 2026Article
- Phage isolation and functional characterization reveal strong antibiofilm activity againstFrontiers in cellular and infection microbiology · 2026Article
- Antibiofilm and antibacterial effects ofBiomaterial investigations in dentistry · 2026Article
- BacQuant: A Scalable Automated Image Processing Pipeline for Quantifying Biofilm Aggregates.microPublication biology · 2026Article
- Differential antimicrobial activity of two LL37 derivatives against periodontal bacteria, including enhanced efficacy againstFrontiers in microbiology · 2026Article
- Heteromultivalent Nanogels as Highly Potent Inhibitors of Pseudomonas Aeruginosa.Angewandte Chemie (International ed. in English) · 2025Article
- Article
- In Vitro Assessment of a Novel Piranha-Passivated Dental Implant Surface Against Oral Biofilm Formation.Clinical oral implants research · 2025Article
- Role of Nurses in Enhancing Removable Dental Prostheses Hygiene.European journal of dentistry · 2025Article
- Characterizing interactions of Staphylococcus aureus and Escherichia coli in dual-species implant-associated biofilms.NPJ biofilms and microbiomes · 2025Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantifying biofilm formation on surfaces is challenging because traditional microbiological methods, such as total colony-forming units (CFUs), often rely on manual counting. These are laborious, resource intensive techniques, more susceptible to human error. Confocal laser scanning microscopy (CLSM) is a high-resolution technique that allows 3D visualisation of biofilm architecture. In combination with a live/dead stain, it can be used to quantify biofilm viability on both transparent and opaque surfaces. However, there is little consensus on the appropriate methodology to apply in confocal micrograph processing. In this study, we report the development of an image analysis approach to repeatably quantify biofilm viability and surface coverage. We also demonstrate its use for a range of bacterial species and translational applications. This protocol has been created with ease of use and accessibility in mind, to enable researchers who do not specialise in computational techniques to be confident in applying these methods to analyse biofilm micrographs. Furthermore, the simplicity of the method enables the user to adapt it for their bespoke needs. Validation experiments demonstrate the automated analysis is robust and accurate across a range of bacterial species and an improvement on traditional microbiological analysis. Furthermore, application to translational case studies show the automated method is a reliable measurement of biomass and cell viability. This approach will ensure image analysis is an accessible option for those in the microbiology and biomaterials field, improve current detection approaches and ultimately support the development of novel strategies for preventing biofilm formation by ensuring comparability across studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.