ArticleNature communications2021
3D particle averaging and detection of macromolecular symmetry in localization microscopy.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Using iPALM to determine protein organisation in cardiac muscle Z-discs.PLoS computational biology · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Towards improved particle reconstruction for single-molecule localization microscopy using geometric deep learning.Bioinformatics advances · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Detecting continuous structural heterogeneity in single molecule localization microscopy data with a point cloud variational auto-encoder.Scientific reports · 2025Article
- Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body.Nature communications · 2025Article
- Super-resolution microscopy for structural biology.Nature methods · 2025Review
- Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy.Nature communications · 2025Article
- Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.Journal of cell science · 2025Article
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- SuperResNET: Model-Free Single-Molecule Network Analysis Software Achieves Molecular Resolution of Nup96.Advanced intelligent systems (Weinheim an der Bergstrasse, Germany) · 2025Article
- Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- ECLiPSE: a versatile classification technique for structural and morphological analysis of 2D and 3D single-molecule localization microscopy data.Nature methods · 2024Article
- Insights into protein structure using cryogenic light microscopy.Biochemical Society transactions · 2023Review
- Detecting continuous structural heterogeneity in single-molecule localization microscopy data.Scientific reports · 2023Article
- Simulating structurally variable nuclear pore complexes for microscopy.Bioinformatics (Oxford, England) · 2023Article
- Particle fusion of super-resolution data reveals the unit structure of Nup96 in Nuclear Pore Complex.Scientific reports · 2023Article
- Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Ångström-resolution fluorescence microscopy.Nature · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D single particle analysis in localization microscopy which hugely increases signal-to-noise ratio and resolution and enables determining the symmetry groups of macromolecular complexes. Our method does not require a structural template, and handles anisotropic localization uncertainties. We demonstrate 3D reconstructions of DNA-origami tetrahedrons, Nup96 and Nup107 subcomplexes of the nuclear pore complex acquired using multiple single molecule localization microscopy techniques, with their structural symmetry deducted from the data.
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