Evidence map›Paper›PMID 33989327›Full record

ArticlePloS one2021

Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis.

Muhammad Arif, Saif Ul Islam, Saqer S Alotaibi, Ahmed M Elshehawi, Mohamed A A Ahmed, Abdullah M Al-Sadi

RetractedOpen access · goldAbstract readRetracted Publication
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 4 countries.

Muhammad ArifDepartment of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.ORCID 0000-0002-8631-4873
Saif Ul IslamDepartment of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
Saqer S AlotaibiDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia.
Ahmed M ElshehawiDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia.
Mohamed A A AhmedPlant Production Department (Horticulture-Medicinal and Aromatic Plants), Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt.
Abdullah M Al-SadiDepartment of Plant Sciences, College of Agricultural and Marine sciences, Sultan Qaboos University, Alkhound, Oman.
Guangdong Academy of Agricultural Sciences · CNTaif University · SAAlexandria University · EGSultan Qaboos University · OM

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.

Indexed as

AgrobacteriumAnimalsBegomovirusCrop ProductionCrops, AgriculturalDNA, ViralGenome, ViralInsect VectorsNicotianaPlant DiseasesSequence Analysis, DNADNA, Viral

Identifiers

PMID33989327
PMCPMC8121359
OpenAlexW3160627631

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.