ArticleJournal of bone and mineral metabolism2021
FSH modulated cartilage ECM metabolism by targeting the PKA/CREB/SOX9 pathway.
Article in Journal of bone and mineral metabolism, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Associations between the serum follicle-stimulating hormone (FSH) levels and intervertebral disc degeneration severity during the menopausal transition.Journal of orthopaedic surgery and research · 2026Article
- Therapeutic potential of SOX family transcription factors in osteoarthritis.Annals of medicine · 2025Review
- Ferroptosis and Sterol Biosynthesis Dysregulation in Granulosa Cells of Patients with Diminished Ovarian Reserve.Antioxidants (Basel, Switzerland) · 2025Article
- FSH enhances the inflammatory response of macrophages in the knee joint possibly through the NFκB pathway.FEBS open bio · 2025Article
- Bezafibrate alleviates diabetes-induced spermatogenesis dysfunction by inhibiting inflammation and oxidative stress.Heliyon · 2024Article
- Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja.Journal of orthopaedic surgery and research · 2024Article
- Research progress on FSH-FSHR signaling in the pathogenesis of non-reproductive diseases.Frontiers in cell and developmental biology · 2024Review
- Independent Skeletal Actions of Pituitary Hormones.Endocrinology and metabolism (Seoul, Korea) · 2022Article
- The anti-fibrotic agent nintedanib protects chondrocytes against tumor necrosis factor-ɑ (TNF-ɑ)-induced extracellular matrix degradation.Bioengineered · 2022Article
- Irisin alleviates obesity-related spermatogenesis dysfunction via the regulation of the AMPKα signalling pathway.Reproductive biology and endocrinology : RB&E · 2021Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
introductionOsteoarthritis (OA) is a common joint disease characterized by articular cartilage degeneration. The prevalence of OA is higher among women than men, and this prevalence is closely related to menopause. The classic view assumes that the underlying mechanism of postmenopausal OA is attributed to declining estrogen levels. Although follicle-stimulating hormone (FSH) levels become elevated in parallel, the effects of FSH on OA have been poorly explored. The present study aimed to study the effect of FSH on cartilage metabolism.
methodsChondrocyte-like ATDC5 cells were treated with recombinant FSH protein. Then the cell viability was measured using cell counting kit-8 assay. Expressions of crucial factors involved in the extracellular matrix (ECM) metabolic and PKA-CREB-SOX9 pathway were analyzed by western blot, RT-qPCR, and immunofluorescence staining. Intracellular cAMP levels were assessed by ELISA assay. Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) surgery. Adeno-associated virus expressing shRNA against FSHR (AAV-shFSHR) was intra-articular (IA) injected into the OA model animals to specifically knock down FHSR in cartilage. Histological staining and OARSI scores were used to assess the efficacy of AAV-shFSHR injections.
resultsWe found that FSH down-regulated the expression of ECM-related proteins in chondrocyte-like ATDC5 cells. The underlying mechanism is probably associated with regulating PKA/CREB/SOX9 pathway. Besides, blocking FSH signaling via shRNA-mediated downregulation of FSHR in joint tissues effectively delayed the development of posttraumatic OA in mice.
conclusionsOur results collectively indicated that FSH plays an essential role in the pathogenesis of OA and acts as a crucial mediator.
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