Evidence map›Paper›PMID 33987815›Full record

ArticleMolecular biotechnology2021

Tissue Culture- and Selection-Independent Agrobacterium tumefaciens-Mediated Transformation of a Recalcitrant Grain Legume, Cowpea (Vigna unguiculata L. Walp).

Anil Kumar, Manish Sainger, Ranjana Jaiwal, Darshna Chaudhary, Pawan K Jaiwal

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. A novel approach: enhancing marigold (Frontiers in plant science · 2024
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Anil KumarCentre for Biotechnology, M. D. University, Rohtak, 124001, India.
Manish SaingerCentre for Biotechnology, M. D. University, Rohtak, 124001, India.
Ranjana JaiwalDepartment of Zoology, M. D. University, Rohtak, 124001, India.
Darshna ChaudharyCentre for Biotechnology, M. D. University, Rohtak, 124001, India.
Pawan K JaiwalCentre for Biotechnology, M. D. University, Rohtak, 124001, India. jaiwalpawan@rediffmail.com.ORCID http://orcid.org/0000-0003-3143-4103
Maharshi Dayanand University · IN

Funding

University Grants Commission F.No. 42-189/2013 (SR)
6 · The paper itself

Abstract

A simple and generally fast Agrobacterium-mediated transformation system with no tissue culture and selection steps has been developed for the first time in a recalcitrant food legume, cowpea. The approach involves wounding of 1-day-old germinated seeds with a needle or sonication either alone or in combination of vacuum infiltration with A. tumefaciens EH105 (pCAMBIA2301) carrying a β-glucuronidase (GUS) gene (uidA) and a neomycin phosphotransferase (nptII) gene for stable transformation. Sonicated and vacuum infiltrated seedlings showed the highest transient GUS activity in 90% of the explants. The sprouted co-cultured seeds directly established in soil and without selection were allowed to develop into plants which on maturity produced T

Indexed as

Transformation, GeneticAgrobacterium tumefaciensGene Transfer TechniquesGerminationGlucuronidaseMeristemPlants, Genetically ModifiedSeedsTransgenesVignaGlucuronidaseAgrobacterium tumefaciensCowpeaSeedling in-planta transformationSonicationVacuum infiltration

Identifiers

PMID33987815
OpenAlexW3163354722

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.