ArticleScientific reports2021
Detection and quantification of bovine papillomavirus DNA by digital droplet PCR in sheep blood.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 17 citations in OpenAlex.
- Detection of papillomavirus DNA in uterine flushing samples from healthy mares reveals diverse genotypes with differing host ranges.Scientific reports · 2026Article
- Accurate identification of bovine deltapapillomavirus in equine sarcoids by ddPCR.Scientific reports · 2025Article
- Digital droplet PCR-based detection and quantification of ovine papillomavirus DNA from the vaginal virobiota of healthy mares.Scientific reports · 2025Article
- The virome investigation of the globally endangered Eld's deer (Rucervus eldii) on Hainan Island, China.Scientific reports · 2025Article
- Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses.Scientific reports · 2025Article
- Clinico-histopathological and molecular detection of small ruminants' papillomaviruses in Iran.Veterinary medicine and science · 2024Article
- Molecular Detection and Quantification of Ovine Papillomavirus DNA in Equine Sarcoid.Transboundary and emerging diseases · 2024Article
- Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen.Frontiers in veterinary science · 2024Article
- Detection and Quantification ofPathogens (Basel, Switzerland) · 2023Article
- Possible etiological association of ovine papillomaviruses with bladder tumors in cattle.Virus research · 2023Review
- Co-infection of distinct papillomavirus types in a captive North American porcupine.Virology journal · 2023Article
- Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries.Frontiers in veterinary science · 2023Article
- Bovine delta papillomavirus E5 oncoprotein negatively regulates the cGAS-STING signaling pathway in cattle in a spontaneous model of viral disease.Frontiers in immunology · 2022Article
- Molecular Epidemiology of Ovine Papillomavirus Infections Among Sheep in Southern Italy.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly pathogenic bovine papillomaviruses (BPVs) were detected and quantified for the first time using digital droplet polymerase chain reaction (ddPCR) by liquid biopsy in 103 clinically healthy sheep. Overall, ddPCR detected BPVs in 68 blood samples (66%). BPV infection by a single genotype was revealed in 61.8% of the blood samples, and BPV coinfection by double, triple or quadruple genotypes was observed in 38.2% of liquid biopsies. The BPV-2 genotype was most frequently seen in sheep, whereas BPV-1 was the least common. Furthermore, ddPCR was very useful for detection and quantification; the BPV-14 genotype was observed for the first time in ovine species, displaying the highest prevalence in some geographical areas (Apulia). In 42 of the positive samples (61.8%), a single BPV infection was observed, 26 of which were caused by BPV-2 (61.9%) and 7 by BPV-13 (16.7%). BPV-14 was responsible for 7 single infections (16.7%) and BPV-1 for 2 single infections (4.7%). Multiple BPV coinfections were observed in the remaining 26 positive samples (38.2%), with dual BPV-2/BPV-13 infection being the most prevalent (84.6%). BPV infection by triple and quadruple genotypes was also observed in 11.5% and 3.8% of cases, respectively. The present study showed that ddPCR, a biotechnological refinement of conventional PCR, is by far the most sensitive and accurate assay for BPV detection compared to conventional qPCR. Therefore, ddPCR displayed an essential diagnostic and epidemiological value very useful for the identification of otherwise undetectable BPV genotypes as well as their geographical distributions and suggesting that animal husbandry practices contribute to cross-species transmission of BPVs.
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