ArticleMicrobial cell factories2021
Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 6 citations in OpenAlex.
- Uncovering virulence factors inApplied and environmental microbiology · 2023Article
- Transposon-based identification of genes involved in the rimocidin biosynthesis in Streptomyces rimosus M527.World journal of microbiology & biotechnology · 2023Article
- Development of a native-locus dual reporter system for the efficient screening of the hyper-production of natural products inFrontiers in bioengineering and biotechnology · 2023Article
- Screening transcriptional connections inMicrobiology (Reading, England) · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
backgroundIdentifying the regulatory factors that control transcriptional activity is a major challenge of gene expression studies. Here, we describe the application of a novel approach for in vivo identification of regulatory proteins that may directly or indirectly control the transcription of a promoter of interest in Streptomyces.
resultsA method based on the combination of Tn5 minitransposon-driven random mutagenesis and lux reporter genes was applied for the first time for the Streptomyces genus. As a proof of concept, we studied the topA supercoiling-sensitive promoter, whose activity is dependent on unknown regulatory factors. We found that the sco4804 gene product positively influences topA transcription in S. coelicolor, demonstrating SCO4804 as a novel player in the control of chromosome topology in these bacteria.
conclusionsOur approach allows the identification of novel Streptomyces regulators that may be critical for the regulation of gene expression in these antibiotic-producing bacteria.
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