ArticleeLife2021
Permeabilization-free
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Probing molecular diversity and ultrastructure of brain cells with fluorescent aptamers.Nature communications · 2026Article
- A human-on-human assay for detecting anti-myocardial antibodies in patients with myocardial disease.Frontiers in immunology · 2026Article
- Genome-wide identification of the crustacyanin gene family in Neocaridina denticulata sinensis based on comparative genomics and localization analysis.BMC genomics · 2025Article
- Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging.Discover nano · 2025Review
- Article
- Evaluating ultrastructural preservation quality in banked brain tissue.Free neuropathology · 2025Article
- Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex.Nature communications · 2024Article
- Advanced Immunolabeling Method for Optical Volumetric Imaging Reveals Dystrophic Neurites of Dopaminergic Neurons in Alzheimer's Disease Mouse Brain.Molecular neurobiology · 2024Article
- GAUSS-EM, guided accumulation of ultrathin serial sections with a static magnetic field for volume electron microscopy.Cell reports methods · 2024Article
- Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Preserving extracellular space for high-quality optical and ultrastructural studies of whole mammalian brains.Cell reports methods · 2023Article
- Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex.Research square · 2023Article
- Layer-specific distribution and expression pattern of AMPA- and NMDA-type glutamate receptors in the barrel field of the adult rat somatosensory cortex: a quantitative electron microscopic analysis.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain.Nature methods · 2022Article
- Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Measurement of arbitrary scan patterns for correction of imaging distortions in laser scanning microscopy.Biomedical optics express · 2022Article
- Retinal horizontal cells use different synaptic sites for global feedforward and local feedback signaling.Current biology : CB · 2022Article
- Reconstructing neural circuits using multiresolution correlated light and electron microscopy.Frontiers in neural circuits · 2022Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
A dense reconstruction of neuronal synaptic connectivity typically requires high-resolution 3D electron microscopy (EM) data, but EM data alone lacks functional information about neurons and synapses. One approach to augment structural EM datasets is with the fluorescent immunohistochemical (IHC) localization of functionally relevant proteins. We describe a protocol that obviates the requirement of tissue permeabilization in thick tissue sections, a major impediment for correlative pre-embedding IHC and EM. We demonstrate the permeabilization-free labeling of neuronal cell types, intracellular enzymes, and synaptic proteins in tissue sections hundreds of microns thick in multiple brain regions from mice while simultaneously retaining the ultrastructural integrity of the tissue. Finally, we explore the utility of this protocol by performing proof-of-principle correlative experiments combining two-photon imaging of protein distributions and 3D EM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.