ArticleInternational journal of molecular medicine2021
Isoquercitrin protects HUVECs against high glucose‑induced apoptosis through regulating p53 proteasomal degradation.
Article in International journal of molecular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Brown Yar Ko Rice Protects Against Hyperglycemia-Induced Endothelial Injury via Antioxidant and SIRT1 Activation.Scientifica · 2026Article
- The Impact of Liraglutide, a GLP-1 Receptor Agonist, on High Glucose-Induced Inflammation, Apoptosis, Oxidative Stress, and NLRP3 Signaling.Cell biochemistry and biophysics · 2025Article
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- Phytochemical Composition ofAdvances in pharmacological and pharmaceutical sciences · 2025Review
- Perilipin 5 alleviates ferroptosis of cardiomyocytes by targeting USP10-p53-TfR proteasome-dependent degradation.Frontiers in medicine · 2025Article
- The antidepressant effects of kaji-ichigoside F1 via activating PPAR-γ/CX3CR1/Nrf2 signaling and suppressing NF-κB/NLRP3 signaling pathways.Frontiers in pharmacology · 2025Article
- Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from quercetin.Microbial cell factories · 2024Article
- Non-invasive and probeless rapid in-vitro monitoring and quantification of HUVECs counts based on FFT impedimetery.BioImpacts : BI · 2024Article
- The pharmacological mechanism ofHeliyon · 2023Review
- Isoquercitrin Induces Endoplasmic Reticulum Stress and Immunogenic Cell Death in Gastric Cancer Cells.Biochemical genetics · 2023Article
- Isoquercitrin restrains the proliferation and promotes apoptosis of human osteosarcoma cells by inhibiting the Wnt/β-catenin pathway.Journal of bone oncology · 2023Article
- Leaves ofAntioxidants (Basel, Switzerland) · 2022Article
- Programmed Cell Death in Diabetic Nephropathy: A Review of Apoptosis, Autophagy, and Necroptosis.Medical science monitor : international medical journal of experimental and clinical research · 2022Review
- Isoquercitrin Attenuates Osteogenic Injury in MC3T3 Osteoblastic Cells and the Zebrafish Model via the Keap1-Nrf2-ARE Pathway.Molecules (Basel, Switzerland) · 2022Article
- Curcumin, Polydatin and Quercetin Synergistic Activity Protects from High-Glucose-Induced Inflammation and Oxidative Stress.Antioxidants (Basel, Switzerland) · 2022Article
- Vascular Effects of Polyphenols fromPharmaceuticals (Basel, Switzerland) · 2022Article
- Ethyl Acetate Fraction and Isolated Phenolics Derivatives fromPharmaceutics · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High glucose (HG)‑induced endothelial apoptosis serves an important role in the vascular dysfunction associated with diabetes mellitus (DM). It has been reported that isoquercitrin (IQC), a flavonoid glucoside, possesses an anti‑DM effect, but the mechanism requires further investigation. The present study investigated the effect of IQC against HG‑induced apoptosis in human umbilical vein endothelial cells (HUVECs) and explored its molecular mechanism. HUVECs were treated with 5 or 30 mM glucose for 48 h. Endothelial cell viability was monitored using the Cell Counting Kit‑8 assay. Mitochondrial membrane potential was detected by JC‑1 staining. Apoptosis was observed by TUNEL staining and flow cytometry. Western blotting was used for the analysis of apoptosis‑associated proteins Bax, Bcl‑2, cleaved (C)‑caspase3, total‑caspase3, p53 and phosphorylated p53. Reverse transcription‑quantitative PCR was used to analyze the mRNA expression levels of Bax, Bcl‑2 and p53. Immunofluorescence staining was utilized to detect the expression levels and distribution of p53 and ubiquitin specific peptidase 10 (USP10) in HUVECs. The results revealed that IQC significantly attenuated HG‑induced endothelial apoptosis, as shown by decreased apoptotic cells observed by TUNEL, JC‑1 staining and flow cytometry. Moreover, under HG stress, IQC treatment markedly inhibited the increased expression levels of the pro‑apoptotic proteins p53, Bax and C‑caspase3, and increased the expression levels of the anti‑apoptotic protein Bcl‑2 in HUVECs. However, the anti‑apoptotic effect of IQC against HG was partially blunted by increasing p53 protein levels
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