ArticleMolecular biotechnology2021
Development of a PCR Lateral Flow Assay for Rapid Detection of Bacillus anthracis, the Causative Agent of Anthrax.
Article in Molecular biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Polymerase Chain Reaction-Lateral Flow Strip for DetectingAntibiotics (Basel, Switzerland) · 2025Article
- Comparative genomic analysis of Streptococcus suis sequence type 105 and development of a PCR diagnostic tool.PloS one · 2025Article
- Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.Materials today. Bio · 2024Review
- Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens.Scientific reports · 2022Article
- Lateral flow immunoassay for small-molecules detection in phytoproducts: a review.Journal of natural medicines · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis, the causative agent of anthrax is one of the most potent listed biological warfare agents. The conventional microbiological methods of its detection are labor intensive and time consuming, whereas molecular assays are fast, sensitive and specific. PCR is one of the most reliable diagnostic tools in molecular biology. The combination of PCR with lateral flow strips can reduce the diagnostic/detection time. It gives an alternative to gel electrophoresis and offers easy and clear interpretation of results. In the present study, a PCR Lateral flow (PCR-LF) assay targeting cya gene present on pXO1 plasmid of B. anthracis has been developed. The forward and reverse primers were tagged with 6-carboxyflourescein (6-FAM) and biotin, respectively, at 5' end. The dual labeled PCR products were detected using lateral flow (LF) strips developed in this study. The PCR-LF assay could detect ≥ 5 pg of genomic DNA and ≥ 500 copies of target DNA harboured in a recombinant plasmid. The assay was able to detect as few as 10
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