ArticleFrontiers in plant science2021
Visualizing the Integrity of Chloroplast Envelope by Rhodamine and Nile Red Staining.
Article in Frontiers in plant science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Technological advances in imaging and modelling of leaf structural traits: a review of heat stress in wheat.Journal of experimental botany · 2026Review
- Investigations of Diclofenac Sorption on Intact and ModifiedInternational journal of molecular sciences · 2026Article
- Moderate levels of dissolved iron stimulate cellular growth and increase lipid storage in Symbiodinium sp.Journal of phycology · 2025Article
- LTD coordinates chlorophyll biosynthesis and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN transport.The Plant cell · 2025Article
- Oxygenating respiratoid biosystem for therapeutic cell transplantation.Nature communications · 2024Article
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- Chloroplast Isolation in Agavaceas.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Live-cell imaging of the chloroplast outer envelope membrane using fluorescent dyes.Plant direct · 2022Article
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Authors and funding
6 authors.
Funding
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Abstract
Chloroplasts are essential organelles in plant cells with many important functions. Chloroplasts isolated by Percoll density gradient centrifugation are widely used in the study of chloroplasts. The intactness of isolated chloroplasts is necessary for many of the experiments. In the past, those isolated chloroplasts were either simply believed to be intact or had to be analyzed by indirect biochemical methods. Here we show a new method to check the intactness of isolated chloroplasts by staining their envelope with fluorescent dyes, Rhodamine or Nile red, and then observing them with a fluorescence microscope. With this method, broken chloroplasts and intact chloroplasts can be distinguished easily and their integrity can be checked in a few minutes. Results of this method agreed well with those of biochemical methods. Moreover, we have also found that sometimes the middle layer chloroplasts from the Percoll gradient centrifugation could be mostly broken, which could cause mistakes in the experiment. With our method, this problem can be easily found. This chloroplast envelope staining method can be used in the preparation of isolated chloroplasts to ensure the intactness.
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