ArticleCommunications biology2021
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 83 citations in OpenAlex.
- Promiscuous fungal peroxygenase fromiScience · 2026Article
- Engineering peroxygenases for pharmaceutical applications: recent advances and future perspectives.Chemical science · 2026Review
- Unspecific Peroxygenase-Based Chemo-Enzymatic Synthesis of Amino Alcohols for Pharmaceutical Preparation.Chembiochem : a European journal of chemical biology · 2026Article
- Structure, characterisation and application of an unspecific peroxygenase fromRSC chemical biology · 2026Article
- Empowering Precision C-H Functionalization: Advances in Peroxygenase Engineering.Angewandte Chemie (International ed. in English) · 2026Review
- Comprehensive assessment of fungal peroxygenases for the selective synthesis of 25-hydroxyvitamins DBiotechnology for biofuels and bioproducts · 2026Article
- N-Glycosylation Influences the Heterologous Expression of an Unspecific Peroxygenase From Marasmius rotula in Saccharomyces cerevisiae.Microbial biotechnology · 2026Article
- Enhanced secretion of an Agrocybe aegerita peroxygenase variant in K. phaffii using the native signal peptide.AMB Express · 2026Article
- Combination of a UPO-Based Epoxidation With a Subsequent Ring-Opening Reaction for the Synthesis of Amino Alcohols.Chembiochem : a European journal of chemical biology · 2026Article
- Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.ACS synthetic biology · 2026Article
- Efficient Expression of Lactone Hydrolase Cr2zen for Scalable Zearalenone Degradation inToxins · 2025Article
- Designing an oxidase toolbox for site-directed oxidation of taxanes.Nature communications · 2025Article
- Exploring Unspecific Peroxygenase Selectivity with Diverse Hydrocarbon Substrates.ChemistryOpen · 2025Article
- Unravelling the Functional Diversity of Type III Polyketide Synthases in Fungi.Angewandte Chemie (International ed. in English) · 2025Article
- Biocatalytic Conversion of Furans into Pyrrolinones Using a Class I Unspecific Peroxygenase.ACS catalysis · 2025Article
- YTK Display-and-Secrete: Screening for Optimal Protein Secretion Elements inACS synthetic biology · 2025Article
- The multipurpose cell factory Aspergillus niger can be engineered to produce hydroxylated collagen.Biotechnology for biofuels and bioproducts · 2025Article
- Computationally Designed Peroxygenases That Exhibit Diverse and Selective Terpene Oxyfunctionalization.ACS catalysis · 2025Article
- Illuminating Glucomannan Synthases To Explore Cell Wall Synthesis Bottlenecks.ACS synthetic biology · 2025Article
- Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal unspecific peroxygenases (UPOs) represent an enzyme class catalysing versatile oxyfunctionalisation reactions on a broad substrate scope. They are occurring as secreted, glycosylated proteins bearing a haem-thiolate active site and rely on hydrogen peroxide as the oxygen source. However, their heterologous production in a fast-growing organism suitable for high throughput screening has only succeeded once-enabled by an intensive directed evolution campaign. We developed and applied a modular Golden Gate-based secretion system, allowing the first production of four active UPOs in yeast, their one-step purification and application in an enantioselective conversion on a preparative scale. The Golden Gate setup was designed to be universally applicable and consists of the three module types: i) signal peptides for secretion, ii) UPO genes, and iii) protein tags for purification and split-GFP detection. The modular episomal system is suitable for use in Saccharomyces cerevisiae and was transferred to episomal and chromosomally integrated expression cassettes in Pichia pastoris. Shake flask productions in Pichia pastoris yielded up to 24 mg/L secreted UPO enzyme, which was employed for the preparative scale conversion of a phenethylamine derivative reaching 98.6 % ee. Our results demonstrate a rapid, modular yeast secretion workflow of UPOs yielding preparative scale enantioselective biotransformations.
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