ArticleThe journal of physical chemistry. B2021
Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.
Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- A fluorescent-protein spin qubit.Nature · 2025Article
- Molecular dynamics guided identification of a brighter variant of superfolder Green Fluorescent Protein with increased photobleaching resistance.Communications chemistry · 2025Article
- Impact of triplet state population on GFP-type fluorescence and photobleaching.Biology of the cell · 2025Article
- Exploring the Versatile Uses of Triplet States: Working Principles, Limitations, and Recent Progress in Phosphorescence, TADF, and TTA.ACS applied optical materials · 2024Review
- Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity.Nature biotechnology · 2024Article
- Sequential Two-Photon Delayed Fluorescence Anisotropy for Macromolecular Size Determination.The journal of physical chemistry. B · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Modulating fluorescent protein emission holds great potential for increasing readout sensitivity for applications in biological imaging and detection. Here, we identify and engineer optically modulated yellow fluorescent proteins (EYFP, originally 10C, but renamed EYFP later, and mVenus) to yield new emitters with distinct modulation profiles and unique, optically gated, delayed fluorescence. The parent YFPs are individually modulatable through secondary illumination, depopulating a long-lived dark state to dynamically increase fluorescence. A single point mutation introduced near the chromophore in each of these YFPs provides access to a second, even longer-lived modulatable dark state, while a different double mutant renders EYFP unmodulatable. The naturally occurring dark state in the parent YFPs yields strong fluorescence modulation upon long-wavelength-induced dark state depopulation, allowing selective detection at the frequency at which the long wavelength secondary laser is intensity modulated. Distinct from photoswitches, however, this near IR secondary coexcitation repumps the emissive S
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.