ArticleDevelopmental neurobiology2021
Ultrastructural analysis of dendritic spine necks reveals a continuum of spine morphologies.
Article in Developmental neurobiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Curvature-based machine-learning method for automated segmentation of dendritic spines.Biophysical journal · 2026Article
- Analysis of Dendritic Specializations in Two Classes of Kenyon Cells in the Mushroom Body of the Adult Honeybee, Apis mellifera.The Journal of comparative neurology · 2026Article
- Sleep deprivation alters hippocampal dendritic spines in a contextual fear memory engram.Scientific reports · 2026Article
- Spine-neck electrical bottlenecks tune temporal precision and inhibitory gating in cortical pyramidal neurons: A connectomics-based biophysical study.bioRxiv : the preprint server for biology · 2026Article
- 3D dendritic spines shape descriptors for efficient classification and morphology analysis in control and Alzheimer's disease modeling neurons.Bioinformatics (Oxford, England) · 2026Article
- Frenet-Serret Frame-Based Decomposition for Part Segmentation of 3-D Curvilinear Structures.IEEE transactions on medical imaging · 2025Article
- Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation.Lab animal · 2025Article
- Change of Spiny Neuron Structure in the Basal Ganglia Song Circuit and Its Regulation by miR-9 during Song Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- VSOT: volume-surface optimization for accurate ultrastructure analysis of dendritic spines.Bioinformatics (Oxford, England) · 2025Article
- Article
- Structural neural plasticity evoked by rapid-acting antidepressant interventions.Nature reviews. Neuroscience · 2025Review
- Calcium signaling in postsynaptic mitochondria: mechanisms, dynamics, and role in ATP production.Frontiers in molecular neuroscience · 2025Review
- Distribution of spine classes shows intra-neuronal dendritic heterogeneity in mouse cortex.Neurophotonics · 2025Article
- Cofilin linked to GluN2B subunits of NMDA receptors is required for behavioral sensitization by changing the dendritic spines of neurons in the caudate and putamen after repeated nicotine exposure.Behavioral and brain functions : BBF · 2024Article
- Cryo-EM structure of human class C orphan GPCR GPR179 involved in visual processing.Nature communications · 2024Article
- A modular framework for multi-scale tissue imaging and neuronal segmentation.Nature communications · 2024Article
- Structural changes of CA1 pyramidal neurons after stroke in the contralesional hippocampus.Brain pathology (Zurich, Switzerland) · 2024Article
- Intracellular magnesium optimizes transmission efficiency and plasticity of hippocampal synapses by reconfiguring their connectivity.Nature communications · 2024Article
- Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders.Journal of extracellular vesicles · 2024Article
- Isochronic development of cortical synapses in primates and mice.Nature communications · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Dendritic spines are membranous protrusions that receive essentially all excitatory inputs in most mammalian neurons. Spines, with a bulbous head connected to the dendrite by a thin neck, have a variety of morphologies that likely impact their functional properties. Nevertheless, the question of whether spines belong to distinct morphological subtypes is still open. Addressing this quantitatively requires clear identification and measurements of spine necks. Recent advances in electron microscopy enable large-scale systematic reconstructions of spines with nanometer precision in 3D. Analyzing ultrastructural reconstructions from mouse neocortical neurons with computer vision algorithms, we demonstrate that the vast majority of spine structures can be rigorously separated into heads and necks, enabling morphological measurements of spine necks. We then used a database of spine morphological parameters to explore the potential existence of different spine classes. Without exception, our analysis revealed unimodal distributions of individual morphological parameters of spine heads and necks, without evidence for subtypes of spines. The postsynaptic density size was strongly correlated with the spine head volume. The spine neck diameter, but not the neck length, was also correlated with the head volume. Spines with larger head volumes often had a spine apparatus and pairs of spines in a post-synaptic cell contacted by the same axon had similar head volumes. Our data reveal a lack of morphological subtypes of spines and indicate that the spine neck length and head volume must be independently regulated. These results have repercussions for our understanding of the function of dendritic spines in neuronal circuits.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.