ArticleMethods in molecular biology (Clifton, N.J.)2021
Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
5 citing papers in PubMed.
- RAS G-domains allosterically contribute to the recognition of lipid headgroups and acyl chains.The Journal of cell biology · 2024Article
- Membrane-Driven Dimerization of the Peripheral Membrane Protein KRAS: Implications for Downstream Signaling.International journal of molecular sciences · 2024Review
- Direct Modulators of K-Ras-Membrane Interactions.ACS chemical biology · 2023Article
- RAS nanoclusters are cell surface transducers that convert extracellular stimuli to intracellular signalling.FEBS letters · 2023Review
- Lipid Profiles of RAS Nanoclusters Regulate RAS Function.Biomolecules · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The function of lipid-anchored small GTPases RAS proteins is mostly compartmentalized to the plasma membrane (PM). Complex biophysical interactions between the C-terminal membrane-anchoring domains of RAS isoforms and PM lipids drive spatial segregation of RAS molecules in the formation of nanometer-sized domains, termed as nanoclusters. These RAS/lipid proteolipid nano-assemblies are the main sites for efficient effector recruitment and signal transduction. Here, we describe a super-resolution imaging method to quantify the nanometer-sized nanoclustering of RAS over a length scale between 8 and 240 nm on intact PM sheets of mammalian cells. Detailed molecular spatial distribution parameters, including the extent of nanoclustering, average cluster size, clustered fraction, and population distribution can be obtained by the univariate spatial distribution analysis. Intermolecular associations between different RAS isoforms, RAS and various PM lipids, as well as RAS and diverse effectors can be quantified via bivariate co-localization analysis.
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Registered trials
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