Evidence map›Paper›PMID 33977473›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Production and Membrane Binding of N-Terminally Acetylated, C-Terminally Farnesylated and Carboxymethylated KRAS4b.

Simon Messing, Constance Agamasu, Matt Drew, Caroline J DeHart, Andrew G Stephen, William K Gillette

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.1field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. The Nanodisc System for Investigating Protein-Lipid Interactions.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Simon MessingNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Constance AgamasuNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Matt DrewNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Caroline J DeHartNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Andrew G StephenNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
William K GilletteNCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. gillettew@mail.nih.gov.
Frederick National Laboratory for Cancer Research · US

Funding

NCI NIH HHS HHSN261200800001E
6 · The paper itself

Abstract

Recombinant mammalian proteins are routinely produced in E. coli and thus lack post-translational modifications. KRAS4b is processed at both the N- and C-terminus, resulting in an acetylation of the N-terminus (at Thr, after aminopeptidase removal of the original N-term Met) and farnesylation/carboxymethylation of the C-terminal Cys (after proteolytic cleavage of the original C-terminal three amino acids, Val-Iso-Met). Processing of KRAS enables it to associate with the plasma membrane and fulfill its function in cell signaling. We describe here the production of recombinant KRAS4b from our modified baculovirus/insect cell expression system that accurately incorporates these in vivo modifications to allow experiments that anchor KRAS4b to membrane mimetics (e.g., nanodiscs and liposomes).

Indexed as

Protein PrenylationProtein Processing, Post-TranslationalAcetylationAmino Acid SequenceCell MembraneHumansMethylationProto-Oncogene Proteins p21(ras)Recombinant ProteinsKRAS protein, humanProto-Oncogene Proteins p21(ras)Recombinant ProteinsCarboxymethylationFarnesylationKRASMembrane bindingNanodiscsN-terminal acetylationProtein purification

Identifiers

PMID33977473
OpenAlexW3161535951

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.