Trial reportScientific reports2021
Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography.
Trial report in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Polarization-Sensitive Holotomography for Multidimensional Label-Free Imaging and Characterization of Lipid Droplets in Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- On-Chip Polarization Light Microscopy.Biosensors · 2025Article
- Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography.Light, science & applications · 2024Article
- Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research.Discover nano · 2024Review
- Ptychographic lens-less birefringence microscopy using a mask-modulated polarization image sensor.Scientific reports · 2023Article
- Improved polarized light microscopic detection of gouty crystals via dissolution with formalin and ethylenediamine tetraacetic acid.Scientific reports · 2023Article
- Methodological advances in necroptosis research: from challenges to solutions.Journal of the National Cancer Center · 2022Article
- Static and Dynamic Biomaterial Engineering for Cell Modulation.Nanomaterials (Basel, Switzerland) · 2022Review
- A glance into the future of gout.Therapeutic advances in musculoskeletal disease · 2022Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.