ArticleNature communications2021
A minimalistic cyclic ice-binding peptide from phage display.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Antifreezing hydrogels for biomedical applications from design strategies to emerging multifunctionality.Bioactive materials · 2026Review
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- Recent Advances in Antifreeze Peptide Preparation: A Review.Molecules (Basel, Switzerland) · 2024Review
- An enzymatic continuous-flow reactor based on a pore-size matching nano- and isoporous block copolymer membrane.Nature communications · 2024Article
- Review
- Generating Ice-Binding Protein-Polymer Bioconjugates.Methods in molecular biology (Clifton, N.J.) · 2024Article
- High Molecular Weight Polyproline as a Potential Biosourced Ice Growth Inhibitor: Synthesis, Ice Recrystallization Inhibition, and Specific Ice Face Binding.Biomacromolecules · 2023Article
- Anionic Synthetic Polymers Prevent Bacteriophage Infection.Journal of the American Chemical Society · 2023Article
- Microcurvature Controllable Metal-Organic Framework Nanoagents Capable of Ice-Lattice Matching for Cellular Cryopreservation.JACS Au · 2023Article
- Synthesis of poly(vinyl alcohol) by blue light bismuth oxide photocatalysed RAFT. Evaluation of the impact of freeze/thaw cycling on ice recrystallisation inhibition.Polymer chemistry · 2022Article
- Degradable Polyampholytes from Radical Ring-Opening Copolymerization Enhance Cellular Cryopreservation.ACS macro letters · 2022Article
- Minimalistic ice recrystallisation inhibitors based on phenylalanine.Chemical communications (Cambridge, England) · 2022Article
- Gold(I)-Mediated Rapid Cyclization of Propargylated Peptides via Imine Formation.Journal of the American Chemical Society · 2022Article
- Ice Recrystallization Inhibition by Amino Acids: The Curious Case of Alpha- and Beta-Alanine.The journal of physical chemistry letters · 2022Article
- Inhibition of Ice Recrystallization by Nanotube-Forming Cyclic Peptides.Biomacromolecules · 2022Article
- Chemical approaches to cryopreservation.Nature reviews. Chemistry · 2022Review
- Discovery of phosphotyrosine-binding oligopeptides with supramolecular target selectivity.Chemical science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Developing molecules that emulate the properties of naturally occurring ice-binding proteins (IBPs) is a daunting challenge. Rather than relying on the (limited) existing structure-property relationships that have been established for IBPs, here we report the use of phage display for the identification of short peptide mimics of IBPs. To this end, an ice-affinity selection protocol is developed, which enables the selection of a cyclic ice-binding peptide containing just 14 amino acids. Mutational analysis identifies three residues, Asp8, Thr10 and Thr14, which are found to be essential for ice binding. Molecular dynamics simulations reveal that the side chain of Thr10 hydrophobically binds to ice revealing a potential mechanism. To demonstrate the biotechnological potential of this peptide, it is expressed as a fusion ('Ice-Tag') with mCherry and used to purify proteins directly from cell lysate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.