Evidence map›Paper›PMID 33975694›Full record

ArticleAnalytica chimica acta2021

Integration of sample preparation with RNA-Amplification in a hand-held device for airborne virus detection.

Xiao Jiang, Julia C Loeb, Maohua Pan, Trevor B Tilly, Arantza Eiguren-Fernandez, John A Lednicky, Chang-Yu Wu, Z Hugh Fan

Open access · greenAbstract read
In one paragraph

Article in Analytica chimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Development of a Platform forAerosol science and technology : the journal of the American Association for Aerosol Research · 2026
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  2. Article
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  4. Review
  5. Concentrating viable airborne pathogens using a virtual impactor with a compact water-based condensation air sampler.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2024
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Xiao JiangJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, P.O. Box 116131, Gainesville, FL, 32611, USA.
Julia C LoebDepartment of Environmental and Global Health, and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
Maohua PanDepartment of Environmental Engineering Sciences, Engineering School of Sustainable Infrastructure and Environment, University of Florida, Gainesville, FL, USA.
Trevor B TillyDepartment of Environmental Engineering Sciences, Engineering School of Sustainable Infrastructure and Environment, University of Florida, Gainesville, FL, USA.
Arantza Eiguren-FernandezAerosol Dynamics Inc., Berkeley, CA, USA.
John A LednickyDepartment of Environmental and Global Health, and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA. Electronic address: jlednicky@phhp.ufl.edu.
Chang-Yu WuDepartment of Environmental Engineering Sciences, Engineering School of Sustainable Infrastructure and Environment, University of Florida, Gainesville, FL, USA. Electronic address: cywu@ufl.edu.
Z Hugh FanJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, P.O. Box 116131, Gainesville, FL, 32611, USA; Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA; Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL, 32611, USA. Electronic address: hfan@ufl.edu.
University of Florida · USAerosol Dynamics (United States) · US

Funding

Multiplexed Airborne Virus Collection and Detection at the Point-of-CareR01AI158868 · NIAID · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2021 to 2025
$1.6M
NIAID NIH HHS R01 AI158868
6 · The paper itself

Abstract

Aerosol transmission is one of the three major transmission routes of respiratory viruses. However, the dynamics and significance of the aerosol transmission route are not well understood, partially due to the lack of rapid and efficient tools for on-the-spot detection of airborne viruses. We report a hand-held device that integrates a 3D-printed sample preparation unit with a laminated paper-based RNA amplification unit. The sample preparation unit features an innovative reagent delivery scheme based on a ball-based valve capable of storing and delivering reagents through the rotation of the unit without manual pipetting, while the paper-based unit enables RNA enrichment and reverse transcription loop-mediated isothermal amplification (RT-LAMP). We have determined the detection limit of the integrated sample-preparation/amplification device (SPAD) at 1 TCID

Indexed as

Influenza A virusInfluenza A Virus, H1N1 SubtypeMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRNARNAAirborneInfluenza virusIsothermal amplificationPaper microfluidicsSample preparationValve

Identifiers

PMID33975694
PMCPMC8325109
OpenAlexW3159703094

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.