ArticleInternational journal of medical microbiology : IJMM2021
Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.
Article in International journal of medical microbiology : IJMM, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- The carbon starvation-inducible lipoprotein (Slp) influences differential adherence of Escherichia coli O157:H7 at the bovine rectoanal junction.PLoS pathogens · 2026Article
- Distinct Tissue Localization and Transcriptional Profiles of Shiga Toxin-ProducingInternational journal of microbiology · 2026Article
- Comparative genomics of Shiga toxin-producingFrontiers in veterinary science · 2025Article
- Investigating the adherence factors ofMicrobiology spectrum · 2024Article
- Host-Pathogen Interactions during Shiga Toxin-ProducingMicroorganisms · 2024Review
- Characterization of clumpy adhesion ofMicrobiology spectrum · 2024Article
- Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B.Scientific reports · 2023Article
- Comparative Transcriptome Analysis of Shiga Toxin-ProducingMicroorganisms · 2023Article
- Shiga-Toxin-Producing Strains ofMicroorganisms · 2023Article
- Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-ProducingMicroorganisms · 2023Article
- Diversity in Genetic Regulation of Bacterial Fimbriae Assembled by the Chaperone Usher Pathway.International journal of molecular sciences · 2022Review
- Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles.International journal of nanomedicine · 2021Article
- Understanding the transmission dynamics ofPeerJ · 2021Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Super-shed (SS) Escherichia coli O157 (E. coli O157) demonstrate a strong, aggregative, locus of enterocyte effacement (LEE)-independent adherence phenotype on bovine recto-anal junction squamous epithelial (RSE) cells, and harbor polymorphisms in non-LEE-adherence-related loci, including in the type 1 fimbriae operon. To elucidate the role of type 1 fimbriae in strain- and host-specific adherence, we evaluated the entire Fim operon (FimB-H) and its adhesion (FimH) deletion mutants in four E. coli O157 strains, SS17, SS52, SS77 and EDL933, and evaluated the adherence phenotype in bovine RSE and human HEp-2 adherence assays. Consistent with the prevailing dogma that fimH expression is genetically switched off in E. coli O157, the ΔfimHSS52, ΔfimB-HSS52, ΔfimB-HSS17, and ΔfimHSS77 mutants remained unchanged in adherence phenotype to RSE cells. In contrast, the ΔfimHSS17 and ΔfimB-HSS77 mutants changed from a wild-type strong and aggregative, to a moderate and diffuse adherence phenotype, while both ΔfimHEDL933 and ΔfimB-HEDL933 mutants demonstrated enhanced binding to RSE cells (p < 0.05). Additionally, both ΔfimHSS17 and ΔfimHEDL933 were non-adherent to HEp-2 cells (p < 0.05). Complementation of the mutant strains with their respective wild-type genes restored parental phenotypes. Microscopy revealed that the SS17 and EDL933 strains indeed carry type 1 fimbriae-like structures shorter than those seen in uropathogenic E. coli. Taken together, these results provide compelling evidence for a strain and host cell type-dependent role of fimH and the fim operon in E. coli O157 adherence that needs to be further evaluated.
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