ArticlePoultry science2021
Research Note: Modified serum fluorescein isothiocyanate dextran (FITC-d) assay procedure to determine intestinal permeability in poultry fed diets high in natural or synthetic pigments.
Article in Poultry science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Influence of amino acid-deficient diets with and without wheat middlings inclusion on the horizontal transmission of Histomonas meleagridis in turkey poults.Poultry science · 2026Article
- Comparative effects of mannanoligosaccharides, fructooligosaccharides, and potato resistant starch on intestinal integrity, antioxidant status, and cecal microbiota in commercial laying hens.Veterinary world · 2026Article
- Mitigation of oxidative stress in chicken intestinal epithelial cells by functional nutrients.Journal of animal science and technology · 2026Article
- Physicochemical characterization and 16S rRNA analysis of a direct-fed microbial from calf ruminal fluid and its protective effect on Sprague-Dawley rat gut barrier function.Translational animal science · 2025Article
- Potential of guar gum as a leaky gut model in broilers: Digestibility, performance, and microbiota responses.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Effects of hydroxychloroquine on the mucosal barrier and gut microbiota during healing of mice colitis.American journal of translational research · 2024Article
- Food, gut barrier dysfunction, and related diseases: A new target for future individualized disease prevention and management.Food science & nutrition · 2023Review
- Centennial Review: Factors affecting the chicken gastrointestinal microbial composition and their association with gut health and productive performance.Poultry science · 2022Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral administration of fluorescein isothiocyanate dextran (FITC-d) has been used as an indicator for intestinal permeability in poultry research for several years. Under healthy conditions, tight junctions in the intestinal wall will not allow the 4-6kDa FITC-d to enter the bloodstream. Detection of FITC-d in serum (1-hour post-oral administration of FITC-d) has proven to be a reliable indicator of leaky gut syndrome (increased intestinal inflammation and disruption of tight junctions). Administration of supplementary phytobiotics in feed, particularly products with high beta-carotene levels or other pigments, has resulted in strong serum background fluorescence, which can render this assay unreliable. To account for this increase in background autofluorescence, the FITC-d assay procedure has been modified to accommodate these particular serum samples by including pre-administration serum collection from each treatment group to remove background fluorescence. The modified FITC-d procedure detailed will allow for analysis of intestinal permeability in pigmented serum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.