ArticlePloS one2021
Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- In vitro effects of Bacillus velezensis strain Mandacaium against Xanthomonas citri pv. glycines: genomic and metabolomic insights.Scientific reports · 2026Article
- Anti-inflammatory effect of aFrontiers in microbiology · 2026Article
- Potential antibacterial, antioxidant and anticancer activities of Nigerian mountain honey in relation to its bacterial flora.Journal, genetic engineering & biotechnology · 2025Article
- Screening and Comparative Genomics of Probiotic Lactic Acid Bacteria from Bee Bread ofMicroorganisms · 2025Article
- Two Strains of EndophyticIndian journal of microbiology · 2024Article
- Complete genome sequence of aquaculture pond isolate,Microbiology resource announcements · 2024Article
- Analysis of antimicrobial biological activity of a marine Bacillus velezensis NDB.Archives of microbiology · 2024Article
- Identification of a New Compound (4-Fluoro-2-Trifluoromethyl Imidazole) Extracted from a New HalophilicIranian journal of biotechnology · 2023Article
- Identification and characterization of SCCmec typing with psm-mec positivity in staphylococci from patients with coagulase-negative staphylococci peritoneal dialysis-related peritonitis.BMC microbiology · 2023Article
- ESKAPEE Pathogen Biofilm Control on Surfaces with ProbioticAntibiotics (Basel, Switzerland) · 2023Review
- Bacterial diversity of stingless bee honey in Yunnan, China: isolation and genome sequencing of a novel acid-resistantFrontiers in bioengineering and biotechnology · 2023Article
- Antagonistic activity ofCurrent research in microbial sciences · 2023Article
- Evaluation of the polyphenolic profile of native Ecuadorian stingless bee honeys (Current research in food science · 2023Article
- Isolation and Identification of a Bacillus sp. from Freshwater Sediment Displaying Potent Activity Against Bacteria and Phytopathogen Fungi.Current microbiology · 2022Article
- Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee's larval food.BMC microbiology · 2022Article
- Complete Genome Sequence of Bacillus velezensis GMEKP1, Isolated from a Natural Bamboo Hive of Stingless Bees.Microbiology resource announcements · 2021Article
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7 authors.
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Abstract
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have reached epidemic proportions globally. Therefore, there is an urgent need for a continuous supply of antibiotics to combat the problem. In this study, bacteria initially identified as species belonging to the Bacillus amyloliquefaciens operational group were re-identified based on the housekeeping gene, gyrB. Cell-free supernatants (CFS) from the strains were used for antimicrobial tests using the agar well diffusion assay against MRSA and various types of pathogenic bacteria. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and physicochemical characteristics of the CFS were determined. Based on gyrB sequence analysis, five strains (PD9, B7, PU1, BP1 and L9) were identified as Bacillus velezensis. The CFS of all B. velezensis strains showed broad inhibitory activities against Gram-negative and -positive as well as MRSA strains. Strain PD9 against MRSA ATCC 33742 was chosen for further analysis as it showed the biggest zone of inhibition (21.0 ± 0.4 mm). The MIC and MBC values obtained were 125 μl/ml. The crude antimicrobial extract showed bactericidal activity and was stable at various temperatures (40-80°C), pH (4-12), surfactants (Tween 20, Tween 80, SDS and Triton X-100) and metal ions (MgCI2, NaCI2, ZnNO3 and CuSO4) when tested. However, the crude extract was not stable when treated with proteinase K. All these properties resembled the characteristics of peptides. The antimicrobial compound from the selected strain was purified by using solvent extraction method and silica gel column chromatography. The purified compound was subjected to High Performance Liquid Chromatography which resulted in a single peak of the anti-MRSA compound being detected. The molecular weight of the anti-MRSA compound was determined by using SDS-PAGE and zymogram. The size of the purified antimicrobial peptide was approximately ~ 5 kDa. The antimicrobial peptide produced from B. velezensis strain PD9 is a promising alternative to combat the spread of MRSA infections in the future.
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