Evidence map›Paper›PMID 33973530›Full record

Observational studyAging2021

Identification of key pathways and genes in carotid atherosclerosis through bioinformatics analysis of RNA-seq data.

Zhongchen Li, Jiheng Hao, Kun Chen, Qunlong Jiang, Peijian Wang, Xiaohui Xing, Jiyue Wang, Yinjiang Zhang, Yilei Xiao, Liyong Zhang

Open access · greenAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Aging, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Zhongchen LiDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Jiheng HaoDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Kun ChenDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Qunlong JiangDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Peijian WangDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Xiaohui XingDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Jiyue WangDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Yinjiang ZhangSchool of Pharmacy, Minzu University of China, Zhongguancun, Beijing 100081, P.R. China.
Yilei XiaoDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Liyong ZhangDepartment of Neurosurgery, Liaocheng People's Hospital, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng 252000, Shandong Province, P.R. China.
Shandong University · CNLiaocheng People's Hospital · CNShandong Provincial Hospital · CNMinzu University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While acknowledging carotid atherosclerosis (CAS) as a risk factor for ischemic stroke, reports on its pathogenesis are scarce. This study aimed to explore the potential mechanism of CAS through RNA-seq data analysis. Carotid intima tissue samples from CAS patients and healthy subjects were subjected to RNA-seq analysis, which yielded, 1,427 differentially expressed genes (DEGs) related to CAS. Further, enrichment analysis (Gene Ontology, KEGG pathway, and MOCDE analysis) was performed on the DEGs. Hub genes identified via the protein-protein interaction network (PPI) were then analyzed using TRRUST, DisGeNET, PaGenBase, and CMAP databases. Results implicated inflammation and immunity in the pathogenesis of CAS. Also, lung disease was associated with CAS. Hub genes were expressed in multiple diseases, mainly regulated by RELA and NFKB1. Moreover, three small-molecule compounds were found via the CMAP database for management of CAS; hub genes served as potential targets. Collectively, inflammation and immunity are the potential pathological mechanisms of CAS. This study implicates CeForanide, Chenodeoxycholic acid, and 0317956-0000 as potential drug candidates for CAS treatment.

Indexed as

Carotid Artery DiseasesCase-Control StudiesCefamandoleChenodeoxycholic AcidComputational BiologyDatasets as TopicFemaleGene Expression RegulationHealthy VolunteersHumansMaleMiddle AgedProtein Interaction MapsRNA-SeqTunica IntimaCefamandoleceforanideChenodeoxycholic Acidbioinformatics analysiscarotid atherosclerosisenrichment analysishub geneRNA-seq

Identifiers

PMID33973530
PMCPMC8148499
OpenAlexW3160128101

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.