Evidence map›Paper›PMID 33973321›Full record

ArticleEnvironmental microbiology2021

Genetic insights into the dark matter of the mammalian gut microbiota through targeted genome reconstruction.

Gabriele Andrea Lugli, Giulia Alessandri, Christian Milani, Alice Viappiani, Federico Fontana, Chiara Tarracchini, Leonardo Mancabelli, Chiara Argentini, Lorena Ruiz, Abelardo Margolles and 3 more

Abstract read
In one paragraph

Article in Environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
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  9. Disclosing the Genomic Diversity among Members of theApplied and environmental microbiology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Gabriele Andrea Lugli *Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Giulia Alessandri *Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Christian MilaniLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.ORCID 0000-0002-5062-3164
Alice ViappianiGenProbio srl, Parma, Italy.
Federico FontanaLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Chiara TarracchiniLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Leonardo MancabelliLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Chiara ArgentiniLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.
Lorena RuizDepartment of Microbiology and Biochemistry, Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, Asturias, 33300, Spain.
Abelardo MargollesDepartment of Microbiology and Biochemistry, Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, Asturias, 33300, Spain.
Douwe van SinderenAPC Microbiome Institute and School of Microbiology, Bioscience Institute, National University of Ireland, Cork, T12YT20, Ireland.
Francesca TurroniLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.ORCID 0000-0001-5363-0231
Marco VenturaLaboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, 43124, Italy.ORCID 0000-0002-4875-4560

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Whole metagenomic shotgun (WMS) sequencing has dramatically enhanced our ability to study microbial genomics. The possibility to unveil the genetic makeup of bacteria that cannot be easily isolated has significantly expanded our microbiological horizon. Here, we report an approach aimed at uncovering novel bacterial species by the use of targeted WMS sequencing. Employing in silico data retrieved from metabolic modelling to formulate a chemically defined medium (CDM), we were able to isolate and subsequently sequence the genomes of six putative novel species of bacteria from the gut of non-human primates.

Indexed as

Gastrointestinal MicrobiomeAnimalsBacteriaGenome, BacterialMetagenomeMetagenomics

Identifiers

PMID33973321
PMCPMC8359967

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.