Modeling and Experimental Validation of Algorithms for Maximum Quantity of Protein to be Immobilized on Solid Supports by Electrostatic Adsorption in the Strategy of Rational Design of Immobilized Derivatives.

Pedro L Arruebo-Rivera, Freddy Castillo-Alfonso, Amanda Troya, Yosberto Cárdenas-Moreno, Patricia Pérez-Ramos, Jorge González-Bacerio, José M Guisán Seijas, Alberto Del Monte-Martínez

Abstract readValidation Study

PubMed Publisher

In one paragraph

Article in The protein journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Immobilization of the Bacillus licheniformis α-Amylase on Azole Functionalized Nanoparticle: More Active, Stable, and Usability.The protein journal · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pedro L Arruebo-RiveraCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Freddy Castillo-AlfonsoCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Amanda TroyaCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Yosberto Cárdenas-MorenoCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Patricia Pérez-RamosInstituto de Farmacia y Alimentos, Universidad de La Habana, San Lázaro y L, Vedado, 10400, Havana, Cuba.

Jorge González-BacerioCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba. jogoba@fbio.uh.cu.ORCID 0000-0002-7155-9165

José M Guisán SeijasDepartamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Campus Cantoblanco, 28049, Madrid, Spain.ORCID 0000-0003-1627-6522

Alberto Del Monte-MartínezCentro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba. adelmonte@fbio.uh.cu.ORCID 0000-0001-9608-5448

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein immobilization by electrostatic adsorption to a support could represent a good option. On the other hand, lysozyme (EC 3.2.1.17) is a little and basic protein. The objective of this work was to test the functionality of the strategy of Rational Design of Immobilized Derivatives for the immobilization by electrostatic adsorption of egg white lysozyme on SP-Sepharose FastFlow support. The RDID

Indexed as

AlgorithmsSoftwareEnzymes, ImmobilizedMuramidaseStatic ElectricityEnzymes, ImmobilizedMuramidaseExperimental validationImmobilization by electrostatic adsorptionMaximum quantity of protein to be immobilizedModeling of algorithmsMost probable configurationProtein loading study

Identifiers

PMID33973097

DOI10.1007/s10930-021-09992-7

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.