ArticleNature neuroscience2021
Integrating barcoded neuroanatomy with spatial transcriptional profiling enables identification of gene correlates of projections.
Article in Nature neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Article
- Selective collateralization of transcriptomically distinct neurons organizes visual-stream output from the primary visual cortex.bioRxiv : the preprint server for biology · 2026Article
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Conserved and specialized features of thalamocortical wiring revealed by single-cell projection mapping in mouse and marmoset.bioRxiv : the preprint server for biology · 2026Article
- SpaHNR: a spatial domain identification method via sparse attention-based hierarchical node representation and multi-view contrastive learning.BMC bioinformatics · 2026Article
- POINTseq: Cell-type-specific barcoding reveals single-cell projection architecture of the mouse dopaminergic system.Neuron · 2026Article
- Review
- BARseq3: a modular system for integrating spatial multi-omics and cellular barcoding in single cells.bioRxiv : the preprint server for biology · 2026Article
- A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic system.bioRxiv : the preprint server for biology · 2026Article
- MAPseq2: a sensitive barcoded connectomics method.bioRxiv : the preprint server for biology · 2026Article
- Spatially resolved in situ profiling of mRNA life cycle at transcriptome scale in intact cells and tissues using STARmap PLUS, RIBOmap and TEMPOmap.Nature protocols · 2026Review
- Organization of mouse prefrontal cortex subnetwork revealed by spatial single-cell multi-omic analysis of SPIDER-Seq.National science review · 2026Article
- Circuit architecture and axonal branching in the efferent auditory system.Hearing research · 2026Review
- BrainConnect: processing brain connectivity and spatial transcriptomics data for integrative analysis.Bioinformatics (Oxford, England) · 2026Article
- Advances in the pathophysiological study of brain development: application of cerebral organoid combined with Spatial omics technology.Stem cell research & therapy · 2026Review
- Uncovering the molecular logic of cortical wiring between neuronal subtypes across development through ligand-receptor inference.Nature communications · 2026Article
- A single-cell spatiotemporal transcriptomic atlas of mouse prefrontal cortex maps dynamics of intratelencephalic neurons during postnatal development.PLoS biology · 2026Article
- Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons.Cell reports methods · 2025Article
- Article
10 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
Abstract
Functional circuits consist of neurons with diverse axonal projections and gene expression. Understanding the molecular signature of projections requires high-throughput interrogation of both gene expression and projections to multiple targets in the same cells at cellular resolution, which is difficult to achieve using current technology. Here, we introduce BARseq2, a technique that simultaneously maps projections and detects multiplexed gene expression by in situ sequencing. We determined the expression of cadherins and cell-type markers in 29,933 cells and the projections of 3,164 cells in both the mouse motor cortex and auditory cortex. Associating gene expression and projections in 1,349 neurons revealed shared cadherin signatures of homologous projections across the two cortical areas. These cadherins were enriched across multiple branches of the transcriptomic taxonomy. By correlating multigene expression and projections to many targets in single neurons with high throughput, BARseq2 provides a potential path to uncovering the molecular logic underlying neuronal circuits.
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