ArticleNature chemistry2021
RNA origami design tools enable cotranscriptional folding of kilobase-sized nanoscaffolds.
Article in Nature chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Programmable Edge-to-Edge Assembly of RNA Nanostructures.ACS nano · 2026Article
- De novo design of RNA pseudoknots with deep learning.bioRxiv : the preprint server for biology · 2026Article
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Tools For Building Artificial Biological Nanostructures.ACS nano · 2026Review
- Construction of synthetic protein-binding non-genetic DNA systems in living cells.Nature chemistry · 2026Article
- Base modifications shift tertiary structure and activity in synthetic RNA origami and a natural ribozyme.Nature communications · 2026Article
- DNA Origami and Their Application in Biosensors.Biosensors · 2026Review
- Label-Free Microfluidic Modulation Spectroscopy Monitors RNA Origami Structure and Stability.Biosensors · 2026Article
- Uncovering Design and Assembly Rules for mRNA-DNA Origami.Nano letters · 2026Article
- Computational approaches for RNA structure prediction and design.Cell reports. Physical science · 2026Article
- Expression of nano-engineered RNA organelles in bacteria.Nature communications · 2026Article
- Designer RNA nanostructures co-transcribed and self-assembled inside human cell nuclei.Nature communications · 2025Article
- Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025Review
- PyFuRNAce: an integrated design engine for RNA origami.Nature communications · 2025Article
- Nucleic Acid Nanoparticles Redefine Traditional Regulatory Terminology: The Blurred Line between Active Pharmaceutical Ingredients and Excipients.ACS nano medicine · 2025Article
- Internal Phase Separation in Synthetic DNA Condensates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- De novo design of RNA and nucleoprotein complexes.bioRxiv : the preprint server for biology · 2025Article
- Structural insights into higher-order natural RNA-only multimers.Nature structural & molecular biology · 2025Article
- Switchable RNA motifs for dynamic transcriptional control of RNA condensates.Nucleic acids research · 2025Article
- Controlling DNA-RNA strand displacement kinetics with base distribution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
RNA origami is a framework for the modular design of nanoscaffolds that can be folded from a single strand of RNA and used to organize molecular components with nanoscale precision. The design of genetically expressible RNA origami, which must fold cotranscriptionally, requires modelling and design tools that simultaneously consider thermodynamics, the folding pathway, sequence constraints and pseudoknot optimization. Here, we describe RNA Origami Automated Design software (ROAD), which builds origami models from a library of structural modules, identifies potential folding barriers and designs optimized sequences. Using ROAD, we extend the scale and functional diversity of RNA scaffolds, creating 32 designs of up to 2,360 nucleotides, five that scaffold two proteins, and seven that scaffold two small molecules at precise distances. Micrographic and chromatographic comparisons of optimized and non-optimized structures validate that our principles for strand routing and sequence design substantially improve yield. By providing efficient design of RNA origami, ROAD may simplify the construction of custom RNA scaffolds for nanomedicine and synthetic biology.
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