ArticleCommunications biology2021
Role of hydrogen bond alternation and charge transfer states in photoactivation of the Orange Carotenoid Protein.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Atomistic simulations reveal the photoactivation mechanism of a carotenoid-binding photoreceptor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Photoactivation Transition State and Dynamical Response of the Orange Carotenoid Protein.The journal of physical chemistry. B · 2025Article
- Two-Photon-Driven Photoprotection Mechanism in Echinenone-Functionalized Orange Carotenoid Protein.Journal of the American Chemical Society · 2025Article
- Engineering hydrogen bonding at tyrosine-201 in the orange carotenoid protein using halogenated analogues.Photosynthesis research · 2025Article
- The Orange Carotenoid Protein Triggers Cyanobacterial Photoprotection by Quenching Bilins via a Structural Switch of Its Carotenoid.Journal of the American Chemical Society · 2024Article
- Roles of ApcD and orange carotenoid protein in photoinduction of electron transport upon dark-light transition in the Synechocystis PCC 6803 mutant deficient in flavodiiron protein Flv1.Photosynthesis research · 2024Article
- Parameterization of a single H-bond in Orange Carotenoid Protein by atomic mutation reveals principles of evolutionary design of complex chemical photosystems.Frontiers in molecular biosciences · 2023Article
- Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization.Nature communications · 2022Article
- Is orange carotenoid protein photoactivation a single-photon process?Biophysical reports · 2022Article
- Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores.Structural dynamics (Melville, N.Y.) · 2022Article
- Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein.Biophysical journal · 2022Article
- Overview of the "Photoreception" session at the 9th Congress of the Russian Photobiological Society: understanding structure and function of photoreceptors.Biophysical reviews · 2022Article
- A synthetic switch based on orange carotenoid protein to control blue-green light responses in chloroplasts.Plant physiology · 2022Article
- Unifying Perspective of the Ultrafast Photodynamics of Orange Carotenoid Proteins fromJACS Au · 2022Article
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Authors and funding
25 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we propose a possible photoactivation mechanism of a 35-kDa blue light-triggered photoreceptor, the Orange Carotenoid Protein (OCP), suggesting that the reaction involves the transient formation of a protonated ketocarotenoid (oxocarbenium cation) state. Taking advantage of engineering an OCP variant carrying the Y201W mutation, which shows superior spectroscopic and structural properties, it is shown that the presence of Trp201 augments the impact of one critical H-bond between the ketocarotenoid and the protein. This confers an unprecedented homogeneity of the dark-adapted OCP state and substantially increases the yield of the excited photoproduct S*, which is important for the productive photocycle to proceed. A 1.37 Å crystal structure of OCP Y201W combined with femtosecond time-resolved absorption spectroscopy, kinetic analysis, and deconvolution of the spectral intermediates, as well as extensive quantum chemical calculations incorporating the effect of the local electric field, highlighted the role of charge-transfer states during OCP photoconversion.
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