Observational studyScientific reports2021
Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples.
Observational study in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Diagnostic Role of Loop-Mediated Isothermal Amplification (LAMP) Assay in Nasal Swabs and Slit Skin Smear Specimens in Pediatric Leprosy.Indian journal of pediatrics · 2026Article
- Advancements in nanobiosensor technologies for in-vitro diagnostics to point of care testing.Heliyon · 2024Review
- Retrospective study and implementation of a low-cost LAMP-turbidimetric assay for screening αPeerJ · 2024Article
- Naked-Eye Detection of LAMP-Produced Nucleic Acids in Saliva Using Chitosan-Capped AuNPs in a Single-Tube Assay.Analytical chemistry · 2023Article
- Challenges and advances in serological and molecular tests to aid leprosy diagnosis.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification.BMC microbiology · 2023Article
- Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2.iScience · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Polymerase chain reaction and loop-mediated isothermal amplification targeting lic13162, lic20239, and lipL32 genes for leptospirosis diagnosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Development of a Loop-Mediated Isothermal Amplification Assay Coupled With a Lateral Flow Dipstick Test for Detection of Myosin Binding Protein C3Frontiers in veterinary science · 2022Article
- Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens.Current research in microbial sciences · 2022Review
- RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.BMC infectious diseases · 2021Article
- Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection ofFrontiers in molecular biosciences · 2021Article
- A review of nutrition in neuropathic pain of leprosy.Therapeutic advances in infectious diseaseReview
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with the advent of physical scarring in patients. This study reports the development of a Loop-mediated isothermal amplification (LAMP) technique for fast, sensitive and specific amplification of 16S rRNA gene of ML DNA for early detection of leprosy in resource-limited areas. Various parameters were optimized to obtain robust and reliable amplification of ML DNA. Blind clinical validation studies were performed which showed that this technique had complete concurrence with conventional techniques. Total absence of amplification of negative control DNA confirmed the specificity of this test. Various visual detection methods viz. colorimetric, turbidity differentiation and bridge flocculation were standardized to establish easy-to-read and rapid diagnosis. This technique eliminates the lack of accuracy and sensitivity in skin smear tests in patients and the requirement for expensive lab equipments and trained technicians. The technique holds promise for further expansion and has the potential to cater to the unmet needs of society for a cheap, highly-sensitive and robust rapid diagnosis of ML.
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