Evidence map›Paper›PMID 33971889›Full record

ArticleJournal of nanobiotechnology2021

Expression and mechanism of exosome-mediated A FOXM1 related long noncoding RNA in gastric cancer.

Yan Zhang, Lin Chen, Xuanting Ye, Zhixiong Wu, Zeyu Zhang, Biaofeng Sun, Hong Fu, Chuangang Fu, Xiaofei Liang, Hong Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 49 citations in OpenAlex.

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  10. The biological role of extracellular vesicles in gastric cancer metastasis.Frontiers in cell and developmental biology · 2024
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  13. Article
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  16. Biomembrane-wrapped gene delivery nanoparticles for cancer therapy.Frontiers in bioengineering and biotechnology · 2023
    Review
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  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Yan Zhang *Department of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China.
Lin Chen *Department of Colorectal Surgery, Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai, 200120, China.
Xuanting Ye *Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Zhixiong WuDepartment of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China.
Zeyu ZhangDepartment of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China.
Biaofeng SunDepartment of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China.
Hong FuDepartment of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China.
Chuangang FuDepartment of Colorectal Surgery, Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai, 200120, China. fugang416@126.com.
Xiaofei LiangHuzhou Lieyuan Medical Laboratory Company Ltd., No. 800, Rujiadian Rd., Huzhou, 313000, China. xfliang86@126.com.
Hong JiangDepartment of Oncology, Tongji Hospital, Tongji University School of Medicine, No. 389, Putuoxincun Rd., Shanghai, 200065, China. jianghong2046@126.com.ORCID http://orcid.org/0000-0003-2550-8401
Tongji University · CNShanghai East Hospital · CNHuzhou University · CNShanghai University of Traditional Chinese Medicine · CN

Funding

National Natural Science Foundation of China 82073033National Natural Science Foundation of China No. 81773275Natural Science Foundation of Jiangxi Province 20181BAB205045Research Project of Shanghai Municipal Commission of Health and Family Planning 201740038the Pudong New District Health and Family Planning Commission Youth Science and Technology Project No. PW2016B-4the Top-level Clinical Discipline Project of Shanghai Pudong No. PWYgf2018-04
6 · The paper itself

Abstract

backgroundForkhead box protein M1 (FOXM1) is an oncogene regulating tumor growth and metastasis. Exosome was suggested to mediate cell communication by delivering active molecules in cancers. However, the existence of FOXM1 in circulating exosomes and the role of exosome FOXM1 in gastric cancer (GC) were not clear. This study aims to investigate the potential role of FOXM1 related long noncoding RNA (FRLnc1) in exosomes in GC.

resultsThe prepared CD63 immunomagnetic beads (CD63-IMB) had the characteristics of good dispersity and high magnetic response. The isolated exosomes were presented with elliptical membranous particles under a transmission electron microscope (TEM), with the particle size of 89.78 ± 4.8 nm. Western blot (WB) results showed that the exosomes were rich in CD9 and CD81. The Dil-labeled exosomes were distributed around cytoplasm and nucleus of cells by imaging flow cytometry (IFC) analysis. The results of quantitative real-time PCR (qRT-PCR) revealed that the FRLnc1 expressions were up-regulated in GC cells, tumor tissues, and serum of GC patients. An obviously up-regulated FRLnc1 expression was found in serum exosomes of GC patients. Up-regulation of FRLnc1 expression was closely correlated to lymph node metastasis (LNM) and TNM stage with the combination of relevant clinicopathological parameter analysis. The in vitro functional analyses demonstrated that FRLnc1 knockdown by RNA interference suppressed cell proliferation and migration in HGC-27 cells, whereas FRLnc1 overexpression promoted cell proliferation and migration in MKN45 cells. After exosome treatment, the FRLnc1 expression was significantly increased in MKN45 cells, and the MKN45 cells showed increased ability of proliferation and migration.

conclusionGC cells-derived exosomes played roles in promoting the growth and metastasis of GC by transporting FRLnc1, suggesting that FRLnc1 in the exosomes may be a potential biomarker for the diagnosis and treatment of GC. The delivery of FRLnc1 by the exosomes may provide a new way for the treatment of GC. Trial registration 2020-KYSB-094. Registered 23 March 2020-Retrospectively registered.

Indexed as

AgedBiomarkers, TumorCell Line, TumorCell MovementCell ProliferationExosomesFemaleForkhead Box Protein M1Gene Expression Regulation, NeoplasticHumansLymphatic MetastasisMaleMiddle AgedRNA InterferenceRNA, Long NoncodingStomach NeoplasmsBiomarkers, TumorForkhead Box Protein M1FOXM1 protein, humanRNA, Long NoncodingBiomarkerExosomesFOXM1Gastric cancerLong non coding RNA

Identifiers

PMID33971889
PMCPMC8111998
OpenAlexW3161167924

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.