ArticleEpidemiology and health2021
The epidemiology of amoebiasis in Thi-Qar Province, Iraq (2015-2020): differentiation of Entamoeba histolytica and Entamoeba dispar using nested and real-time polymerase chain reaction.
Article in Epidemiology and health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
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7 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Prevalence Estimates and Distribution of Entamoeba histolytica and Entamoeba dispar Infections in Several Regions of Thailand: A Systematic Review and Meta-Analysis.The American journal of tropical medicine and hygiene · 2025Pooled it
- Prevalence of intestinal parasitic infections for six years (2015-2020) in Thi-Qar Province, Iraq.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Decision tree-based learning and laboratory data mining: an efficient approach to amebiasis testing.Parasites & vectors · 2025Article
- Molecular identification ofVeterinary world · 2024Article
- Molecular Detection of a PathogenicPolish journal of microbiology · 2024Article
- Prevalence of Giardiasis and Entamoeba Species in Two of the Six Governorates of Kuwait.Journal of parasitology research · 2022Article
- Identification ofIranian journal of parasitologyArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe objective of this study was to evaluate the present status of amoebiasis in Thi-Qar Province in southern Iraq, and to determine the presence of Entamoeba histolytica and Entamoeba dispar with nested and real-time polymerase chain reaction (PCR).
methodsEpidemiological data were obtained from the public health department of the Thi-Qar Health Office (2015-2020). Eighty stool samples were also randomly collected from patients ≤12 year of age with diarrhea at 2 hospitals between the beginning of February 2020 and the end of October 2020. These samples were selected after microscopy to identify the 18S rRNA gene in Entamoeba DNA.
resultsOf the 341,554 cases of intestinal parasitic infections, 38,004 (11.1%) individuals were recorded as having amoebiasis, which accounted for the highest proportion of infections in 2015 (26.1%) and the lowest in 2020 (8.1%). Amoebiasis was distributed among all age groups, with the age group of 5-14 years accounting for the highest proportion (27.3%). In molecular testing, 42 (52.5%) out of 80 samples were positive for the 18S rRNA gene (888 bp). Using nested PCR, E. histolytica (439 bp) was detected in 25 (31.3%) samples and E. dispar (174 bp) in 14 (17.5%), while using real-time PCR, E. histolytica and E. dispar were detected in 28 (35.0%) and 15 (18.8%) samples, respectively.
conclusionsEpidemiological data confirmed that amoebiasis is endemic in this province, and is not limited to certain months. Our study confirms the applicability of molecular identification to detect pathogenic and non-pathogenic Entamoeba to prescribe the appropriate drug.
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