Evidence map›Paper›PMID 33971375›Full record

ArticleBioelectrochemistry (Amsterdam, Netherlands)2021

Growth environment influences B16.F10 mouse melanoma cell response to gene electrotransfer.

L Heller, A Bulysheva, S Arpag, A Sales Conniff, K Kohena, G Shi, N Semenova, R Heller, M Cemazar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioelectrochemistry (Amsterdam, Netherlands), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

L HellerDepartment of Medical Engineering, University of South Florida, Tampa, FL, USA. Electronic address: lheller@usf.edu.
A BulyshevaDepartment of Electrical & Computer Engineering, Old Dominion University, Norfolk, VA, USA.
S ArpagDepartment of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA, USA.
A Sales ConniffDepartment of Medical Engineering, University of South Florida, Tampa, FL, USA.
K KohenaDepartment of Medical Engineering, University of South Florida, Tampa, FL, USA.
G ShiDepartment of Medical Engineering, University of South Florida, Tampa, FL, USA.
N SemenovaCenter for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
R HellerDepartment of Medical Engineering, University of South Florida, Tampa, FL, USA.
M CemazarDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia; Faculty of Health Sciences, University of Primorska, Izola, Slovenia. Electronic address: mcemazar@onko-i.si.
University of South Florida · USOld Dominion University · USEastern Virginia Medical School · USUniversity of Primorska · SI

Funding

DNA-specific pattern recognition receptor activation following DNA electroporationR01CA196796 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI HELLER, LOREE C · 2016 to 2020
$1.6M
NCI NIH HHS R01 CA196796
6 · The paper itself

Abstract

We developed and characterized a 3D collagen hydrogel model for B16.F10 melanoma tumors. Cells in this 3D environment exhibited lower proliferation than cells in the conventional 2D culture environment. Interestingly, the basal expression levels of several genes varied when compared to conventionally grown cells. In each growth environment, a significant number of melanoma cells were transfected by plasmid electroporation (electrotransfer), although expression could only be ascertained on the surface of the 3D constructs. Cellular responses to plasmid entry as demonstrated by pro-inflammatory cytokine and chemokine upregulation varied based on the growth environment, as did the mRNA levels of several putative DNA-specific pattern recognition receptors (DNA sensors). Unexpectedly, when plasmid DNA was delivered while cells where attached in the 2D or 3D environments, the mRNAs of the DNA sensor p204 and the inflammatory mediator TNFα were regulated in cells receiving pulses only. However, we were unable to confirm coordinate upregulation of TNFα and p204 proteins. This study confirms that cell responses differ significantly based on their environment, and demonstrates the difficulty of extending experimental observations between cell environments.

Indexed as

ElectroporationGene Transfer TechniquesAnimalsMelanoma, ExperimentalMicePlasmidsTransfectionCollagen hydrogelsCytokines and chemokinesDNA electroporation or DNA electrotransferDNA sensorsMelanoma cellsPattern recognition receptors

Identifiers

PMID33971375
PMCPMC8237338
OpenAlexW3163717672

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.