Evidence map›Paper›PMID 33970537›Full record

ArticleMicrobiologyOpen2021

Comparison of four extraction methods for the detection of hepatitis B virus DNA in dried blood spot samples.

Cristianne Sousa Bezerra, Moyra Machado Portilho, Cristiane Cunha Frota, Lívia Melo Villar

Open access · goldAbstract readComparative Study
In one paragraph

Article in MicrobiologyOpen, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Cristianne Sousa BezerraViral Hepatitis Laboratory, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz, Rio de Janeiro, Brazil.
Moyra Machado PortilhoViral Hepatitis Laboratory, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz, Rio de Janeiro, Brazil.
Cristiane Cunha FrotaDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil.
Lívia Melo VillarViral Hepatitis Laboratory, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz, Rio de Janeiro, Brazil.ORCID 0000-0001-7644-8969
Fundação Oswaldo Cruz · BRInstituto Federal de Educação, Ciência e Tecnologia do Ceará · BRUniversidade Federal do Ceará · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The dried blood spot (DBS) samples are a useful resource for viral DNA isolation and important in increasing access to HBV diagnosis. However, the choice of the DNA extraction method is crucial for reliable results. We compared the reliability of four DNA extraction methods using DBS samples for the qualitative and quantitative detection of HBV. A panel of serially diluted HBV DNA in whole blood was spotted onto filter paper (Whatman 903 paper and Whatman FTA cards). Four methods were used to extract DNA: QIAamp

Indexed as

Diagnostic Tests, RoutineDNA, ViralDried Blood Spot TestingGenes, ViralHepatitis BHepatitis B virusHumansMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificitySpecimen HandlingDNA, ViralDBSDNA extractionhepatitis BPCRreal-time PCR

Identifiers

PMID33970537
PMCPMC8107022
OpenAlexW3161442731

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.