Evidence map›Paper›PMID 33970520›Full record

ArticleJournal of magnetic resonance imaging : JMRI2021

In Vivo Magnetic Resonance Spectroscopy of Hyperpolarized [1-

Lauren M Smith, Conrad B Pitts, Lanette J Friesen-Waldner, Neetin H Prabhu, Katherine E Mathers, Kevin J Sinclair, Trevor P Wade, Timothy R H Regnault, Charles A McKenzie

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of magnetic resonance imaging : JMRI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Hyperpolarized carbon 13 MRI in liver diseases: Recent advances and future opportunities.Liver international : official journal of the International Association for the Study of the Liver · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Lauren M SmithDepartment of Medical Biophysics, Western University, London, Ontario, Canada.ORCID 0000-0002-5585-966X
Conrad B PittsDepartment of Physiology and Pharmacology, Western University, London, Ontario, Canada.
Lanette J Friesen-WaldnerDepartment of Medical Biophysics, Western University, London, Ontario, Canada.
Neetin H PrabhuDepartment of Physiology and Pharmacology, Western University, London, Ontario, Canada.
Katherine E MathersDepartment of Physiology and Pharmacology, Western University, London, Ontario, Canada.
Kevin J SinclairDepartment of Medical Biophysics, Western University, London, Ontario, Canada.
Trevor P WadeDepartment of Medical Biophysics, Western University, London, Ontario, Canada.
Timothy R H RegnaultDepartment of Physiology and Pharmacology, Western University, London, Ontario, Canada.
Charles A McKenzieDepartment of Medical Biophysics, Western University, London, Ontario, Canada.ORCID 0000-0002-9614-1582
Western University · CA

Funding

Hyperpolarized 13C MRI of Placental Metabolic Abnormalities Resulting from the Western DietU01HD087181 · NICHD · UNIVERSITY OF WESTERN ONTARIO · PI MCKENZIE, CHARLES A, REGNAULT, TIMOTHY RH · 2015 to 2015
$2.8M
NICHD NIH HHS U01 HD087181
6 · The paper itself

Abstract

backgroundAlterations in glycolysis are central to the increasing incidence of non-alcoholic fatty liver disease (NAFLD), highlighting a need for in vivo, non-invasive technologies to understand the development of hepatic metabolic aberrations. PURPOSE: To use hyperpolarized magnetic resonance spectroscopy (MRS) and proton density fat fraction (PDFF) magnetic resonance imaging (MRI) techniques to investigate the effects of a chronic, life-long exposure to the Western diet (WD) in an animal model resulting in NAFLD; to investigate the hypothesis that exposure to the WD will result in NAFLD in association with altered pyruvate metabolism. STUDY TYPE: Prospective. ANIMAL MODEL: Twenty-eight male guinea pigs weaned onto a control diet (N = 14) or WD (N = 14). FIELD STRENGTH/SEQUENCE: 3 T; T1-weighted gradient echo, T2-weighted spin-echo, three-dimensional gradient multi-echo fat-water separation (IDEAL-IQ), and broadband point-resolved spectroscopy (PRESS) chemical-shift sequences. ASSESSMENT: Median PDFF was calculated in the liver and hind limbs. [1- STATISTICAL TESTS: Unpaired Student's t-tests were used to determine differences in measurements between the two diet groups. The Pearson correlation coefficient was calculated to determine correlations between measurements.

resultsLife-long WD consumption resulted in significantly higher liver PDFF and elevated triglyceride content in the liver. The WD group exhibited a decreased TTP for lactate production, and ex vivo analysis highlighted increased liver lactate dehydrogenase (LDH) activity. DATA

conclusionPDFF MRI results suggest differential fat deposition patterns occurring in animals fed a life-long WD characteristic of lean, or lacking excessive subcutaneous fat, NAFLD. The decreased liver lactate TTP and increased ex vivo LDH activity suggest lipid accumulation occurs in association with a shift from oxidative metabolism to anaerobic glycolytic metabolism in WD-exposed livers. LEVEL OF EVIDENCE: 2 TECHNICAL EFFICACY STAGE: 1.

Indexed as

Non-alcoholic Fatty Liver DiseaseAnimalsDiet, WesternGuinea PigsLiverMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleProspective StudiesProtonsPyruvic AcidReproducibility of ResultsProtonsPyruvic Acidcarbon-13fatty liverhyperpolarized MRSmetabolismNAFLDpyruvate

Identifiers

PMID33970520
PMCPMC8516663
OpenAlexW3161218343

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.