ArticleJournal of assisted reproduction and genetics2021
Mural granulosa cells support to maintain the viability of growing porcine oocytes and its developmental competence after insemination.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- A transcriptomic map of ligand-receptor interactions within bovine antral follicles predicts intrafollicular factors for improving oocyte quality.Journal of animal science and biotechnology · 2026Article
- Niacin improves developmental ability by increasing glutathione levels and promoting male pronuclear formation in porcine oocytes grown in vitro.The Journal of reproduction and development · 2026Article
- Factors affecting the intracytoplasmic sperm cell injection outcomes: A meta-analysis of porcine studies.Journal of advanced research · 2025Article
- Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation.Nature communications · 2025Article
- Intrinsic differences in mTOR activity mediates lineage-specific responses to cyclophosphamide in mouse and human granulosa cells.Journal of ovarian research · 2025Article
- Vitamin C enhances theAnimal biotechnology · 2024Article
- Polystyrene nanoplastics induce apoptosis, autophagy, and steroidogenesis disruption in granulosa cells to reduce oocyte quality and fertility by inhibiting the PI3K/AKT pathway in female mice.Journal of nanobiotechnology · 2024Article
- Integrative analysis of transcriptome complexity in pig granulosa cells by long-read isoform sequencing.PeerJ · 2022Article
- Granulosa Cells Improved Mare Oocyte Cytoplasmic Maturation by Providing Collagens.Frontiers in cell and developmental biology · 2022Article
- Current Status of In Vitro Oocyte Growth and Development in Mammals.Reproductive medicine and biologyReview
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. METHODS AND
resultsWhen OGCs were cultured with MGCs for 12 days, there were significant improvement (P < 0.05) in the robustness of gap junctional communication between the oocyte and the granulosa cells (82% vs. 59%), the survival rate of oocytes (57% vs. 39%), and the diameter of survived oocytes (118 μm vs. 112 μm). The rate of oocyte release of OGCs cultured with MGCs on the 12th day (1.9%) was significantly lower than that of OGCs cultured without MGCs (26%). Complete meiotic arrest was maintained in the group with MGCs (100%), while partial resumption of spontaneous meiosis was noticed in the absence of MGCs (10-19%). Furthermore, the presence of MGCs increased the oocyte maturation rate after maturation culture in both 12- and 14-day culture groups (P < 0.05, 85-88%) compared to OGCs cultured without MGCs (48-60%). MGCs also significantly improved the blastocyst formation rate (day 7) after ICSI (P < 0.05).
conclusionsThe data of this study thus shows that the presence of MGCs during in vitro oocyte growth plays a crucial role in supporting the developmental competence of growing porcine oocytes attached to the granulosa cells via enhancement of their viability.
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