Evidence map›Paper›PMID 33969020›Full record

ArticleFrontiers in cardiovascular medicine2021

circSnx12 Is Involved in Ferroptosis During Heart Failure by Targeting miR-224-5p.

Haoyuan Zheng, Lin Shi, Changci Tong, Yunen Liu, Mingxiao Hou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cardiovascular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed, 1 pooled it
6.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.

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  19. Circular RNAs: a small piece in the heart failure puzzle.Functional & integrative genomics · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Haoyuan ZhengLaboratory of Rescue Center of Severe Wound and Trauma Chinese People's Liberation Army, Department of Cardiovascular Surgery, General Hospital of Northern Theater Command of China Medical University, Shenyang, China.
Lin ShiLaboratory of Rescue Center of Severe Wound and Trauma Chinese People's Liberation Army, Emergency Medicine Department of General Hospital of Northern Theater Command, Shenyang, China.
Changci TongLaboratory of Rescue Center of Severe Wound and Trauma Chinese People's Liberation Army, Emergency Medicine Department of General Hospital of Northern Theater Command, Shenyang, China.
Yunen LiuThe Second Affiliated Hospital of Shenyang Medical College, The Veterans General Hospital of Liaoning Province, Shenyang, China.
Mingxiao HouLaboratory of Rescue Center of Severe Wound and Trauma Chinese People's Liberation Army, Department of Cardiovascular Surgery, General Hospital of Northern Theater Command of China Medical University, Shenyang, China.
Chinese People's Liberation Army · CNChina Medical University · CNGeneral Hospital of Shenyang Military Region · CNShenyang Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circular RNA (circRNA) is a subclass of non-coding RNAs that enables the circular transcripts resistant to the exonuclease digestion. Iron homeostasis is essential for the body to maintain normal physiological functions. At present, the relationship among circRNA, iron metabolism and heart failure remains largely unknown. This study aimed to explore the regulatory mechanism of circRNA and iron metabolism in heart failure. We obtained circRNA, miRNA and mRNA data from public databases and built a ceRNA network. The prediction results were verified in the myocardial tissues of pressure overload-induced heart failure mice through the use of histopathological staining methods, iron and malondialdehyde (MDA) measurement tests, quantitative real-time PCR (qRT-PCR), Western blot analysis and luciferase reporter assay. A total of 4 genes related to iron metabolism and oxidative stress were identified, and a ceRNA network involving 7 circRNAs, 7 miRNAs, and 4 mRNAs was constructed using bioinformatics tools. The results of qRT-PCR and Western blot analyses indicated that the expression level of FTH1 was similar with that predicted by bioinformatics analysis. Echocardiographic measurement showed that heart failure mice have lower fractional shortening and ejection fraction. Moreover, the myocardium of heart failure mice displayed obvious fibrosis as well as increased levels of iron and MDA compared to control mice. Besides, circSnx12 could act as an endogenous sponge to bind with miR-224-5p, and the 3'UTR region of FTH1 also had miRNA binding sites. A circRNA-miRNA-mRNA regulatory network was successfully constructed by identifying differentially expressed genes related to iron metabolism. This new approach reveals potential circRNA targets for the treatment of heart failure.

Indexed as

computational biologyferroptosisheart failureRNAuntranslated

Identifiers

PMID33969020
PMCPMC8097164
OpenAlexW3153524743

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.